Biotechnology and Bioprocess Engineering

, Volume 7, Issue 1, pp 6–9 | Cite as

Enhanced virus removal by flocculation and microfiltration

  • Binbing Han
  • Jonathan O. Carlson
  • Scott M. Powers
  • S. Ranil Wickramasinghe


In this work we have investigated the feasibility of virus clearance by flocculation and tangential flow microfiltration. Chinese hamster ovary cell feed streams were spiked with minute virus of mice and then flocculated using cationic polyelectrolytes prior to tangential flow microfiltration. Our results indicate that flocculation prior to microfiltration leads to more than 100 fold clearance of minute virus of mice particles in the permeate. Today, validation of virus clearance is a major concern in the manufacture of biopharmaceutical products. Frequently new unit operations are added simply to validate virus clearance thus increasing the manufacturing cost. The results obtained here suggest that virus clearance can be obtained during tangential flow microfiltration. Since tangential flow microfiltration is frequently used for bioreactor harvesting this could be a low cost method to validate virus clearance.


flocculation hollow fibres membranes microfiltration virus clearance 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering 2002

Authors and Affiliations

  • Binbing Han
    • 1
  • Jonathan O. Carlson
    • 1
  • Scott M. Powers
    • 1
  • S. Ranil Wickramasinghe
    • 1
  1. 1.Department of Chemical and Bioresource EngineeringColorado State UniversityFort CollinsUSA

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