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Enrichment of wheat bran byRhodotorula gracilis through solid-state fermentation

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Abstract

The potential oil-producing yeastRhodotorula gracilis was found to produce higher yields of biomass (13.7 g/L) and lipids (20.3 %) in a nitrogen-limited and economically cheaper medium (molasses without yeast extract) in a submerged fermentation system. But, when the yeast was grown on four different wheat bran media by solid-state fermentation technique, different media combinations affected the percent increase in biomass, protein, oil production, fatty acid profile and degree of saturation and unsaturation. The initial lipid content in the control medium was 3.5 % while in a medium with wheat bran, molasses, and minerals it was 69.8 %. The yeast did not produceα-amylase, amyloglucosidase and cellulolytic enzymes for the breakdown of wheat bran. The yeast produced red carotenoids, a precursor of vitamin B12 and some oligounsaturated fatty acids in the fermented product.

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References

  • Davies R.J.: Yeast oil from cheese whey-process development, pp. 99–145 in Single Cell Oil (R.S. Moreton, Ed.).Longman Scientific Technical, England 1988.

    Google Scholar 

  • Hesseltine G.W.: Solid state fermentations.Biotechnol.Bioeng. 14, 517–532 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Jacob Z.: Selective medium for the production of microbial lipids byRhodotorula gracilis CFR-1.J.Food Sci.Technol. 25, 373–374 (1988).

    Google Scholar 

  • Jacob Z., Krishnamurthy M.N.: Studies on physico-chemical characteristics and fatty acid composition of lipids produced by a strain ofRhodotorula gracilis CFR-1.J.Am.Oil Chem.Soc. (in press) 1990.

  • Kates M.: Simplified procedures for hydrolysis or methanolysis of lipids.J.Lipid Res. 5, 132–135 (1964).

    CAS  Google Scholar 

  • Mandels M., Andreotti R., Roche C.: Measurement of saccharifying cellulose, pp. 21–23 inBiotechnology and Bioengineering Symposium, Vol. VI (E.L. Canden Jr.,M.H. Mandels, E.T. Reese, L.A. Spano, Eds).John Wiley & Sons, New York 1976.

    Google Scholar 

  • Manning G.B., Campbell L.L.: Thermostable alpha-amylase ofBacillus stearothermophilus.J.Biol.Chem. 236, 2952–2957 (1961).

    PubMed  CAS  Google Scholar 

  • Markham R.: A steam distillation apparatus suitable for micro-Kjeldahl analysis.Biochem.J. 36, 790–791 (1942).

    PubMed  CAS  Google Scholar 

  • Miller G.L.: Use of dinitrosalicylic acid reagent for determination of reducing sugars.Analyt.Chem. 31, 426–428 (1959).

    Article  CAS  Google Scholar 

  • Ramakrishna S.V., Childyal N.P., Lonsane B.K., Ahmed S.Y., Sreenivasa Murthy V.: Wheat bran as substrate in solid state fermentation, pp. 267–283 inProc.Nat.Symp.Biotechnology (S.C. Jain Ed.), Chandigarh 1982.

  • Sreekantiah K.R., Jaleel S.A., Rao T.N.R.: Effect of cultural and nutritional variations on certain exoenzymes secreted by fungi.Chem.Mikrobiol.Lebensm. 2, 42–48 (1973).

    CAS  Google Scholar 

  • Swaminathan M., Bhagawan R.K.:Our Food, 6th Ed., pp. 49–50. Ganesh & Co., Madras 1969.

    Google Scholar 

  • Yoon S.H., Rhim J.W., Choi S.Y., Ryu D.D.W., Rhee J.S.: Effect of carbon and nitrogen of lipid production ofRhodotorula gracilis.J.Ferment.Technol. 60, 243–246 (1982).

    CAS  Google Scholar 

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Jacob, Z. Enrichment of wheat bran byRhodotorula gracilis through solid-state fermentation. Folia Microbiol 36, 86–91 (1991). https://doi.org/10.1007/BF02935828

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