Skip to main content
Log in

Characterization of hematopoietic stem and progenitor cells

  • Cellular Immunology
  • Published:
Immunologic Research Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Broxmeyer, H.E.: Biomolecule-cell interactions and the regulation of myelopoiesis. Int. J. Cell Cloning4: 378–405 (1986).

    PubMed  CAS  Google Scholar 

  2. Lajtha, L.G.: Stem cell concepts. Differentiation14: 23–34 (1979).

    Article  PubMed  CAS  Google Scholar 

  3. Wu, A.M.; Till, J.E.; Siminovitch, L.; McCulloch, E.A.: Cytological evidence for a relationship between normal hematopoietic colony-forming cells and cells of the immune system. J. exp. Med.127: 455–463 (1968).

    Article  PubMed  CAS  Google Scholar 

  4. Lemischka, I.; Raulet, D.H.; Mulligan, R.C.: Developmental potential and dynamic behavior of hematopoietic stem cells. Cell45: 917–927 (1986).

    Article  PubMed  CAS  Google Scholar 

  5. Blackett, N.M.; Millard, R.E.: Different recovery patterns of mouse hemopoietic stem cells in response to cytotoxic agents. J. cell. Physiol.89: 473–480 (1976).

    Article  PubMed  CAS  Google Scholar 

  6. Rosendaal, M.; Hodgson, G.S.; Bradley, T.R.: Organization of hemopoietic stem cells: The generation-age hypothesis. Cell Tiss. Kinet.12: 17–29 (1979).

    CAS  Google Scholar 

  7. Hodgson, G.S.; Bradley, T.R.: Properties of hematopoietic stem cells surviving 5-fluorouracil treatment: evidence for a pre-CFU-S cell? Nature, Lond.281: 381–382 (1979).

    Article  CAS  Google Scholar 

  8. Zant, G. van: Studies of hematopoietic stem cells spared by 5-fluorouracil. J. exp. Med.159: 679–690 (1984).

    Article  PubMed  Google Scholar 

  9. Williams, D.A.; Lemischka, I.; Nathan, D.G.; Mulligan, R.C.: Introduction of new genetic material into pluripotent hematopoietic stem cells of the mouse. Nature, Lond.310: 476–480 (1984).

    Article  CAS  Google Scholar 

  10. Till, J.E.; McCulloch, E.A.: A direct measurement of the radiation sensitivity of normal bone marrow cells. Radiat. Res.14: 213–222 (1961).

    Article  PubMed  CAS  Google Scholar 

  11. Trentin, J.J.: Determination of bone marrow stem cell differentiation by stromal hemopoietic inductive microenvironments (HIM). Am. J. Path.65: 621–628 (1971).

    PubMed  CAS  Google Scholar 

  12. Curry, J.L.; Trentin, J.J.; Wolf, N.: Hematopoietic spleen colony studies. II. Erythropoiesis. J. exp. Med.125: 703–720 (1967).

    Article  PubMed  CAS  Google Scholar 

  13. Magli, M.C.; Iscove, N.N.; Odartchenko, N.: Transient nature of early hematopoietic spleen colonies. Nature, Lond.295: 527–529 (1982).

    Article  CAS  Google Scholar 

  14. Spivak, J.L.; Smith, R.R.L.; Ihle, J.N.: Interleukin-3 promotes the in vitro proliferation of murine pluripotent hematopoietic stem cells. J. clin. Invest.76: 1613–1621 (1985).

    Article  PubMed  CAS  Google Scholar 

  15. Lord, B.I.; Molineux, G.; Testa, N.G.; Kelly, M.; Spooncer, E.; Dexter, T.M.: The kinetic response of hemopoietic precursor cells, in vivo, to highly purified, recombinant interleukin-3. Lymphokine Res.5: 97–104 (1986).

    PubMed  CAS  Google Scholar 

  16. Broxmeyer, H.E.; Williams, D.E.; Hangoc, G.; Cooper, S.; Gentile, P.; Shen, R.N.; Ralph, P.; Gillis, S.; Bicknell, D.C.: The opposing actions in vivo on murine myelopoiesis of purified preparations of lactoferrin and the colony-stimulating factors. Blood Cells (in press).

  17. Ploemacher, R.E.; Brons, R.H.C.; Leenen, P.J.M.: Bulk enrichment of transplantable hemopoietic stem cell subsets from lipopolysaccharide-stimu lated murine spleen. Exp. Hematol.15: 154–162 (1987).

    PubMed  CAS  Google Scholar 

  18. Nakahata, T.; Ogawa, M.: Hemopoietic colony-forming cells in umbilical cord blood with extensive capability to generate mono- and multipotential hemopoietic progenitors. J. clin. Invest.70: 1324–1328 (1982).

    Article  PubMed  CAS  Google Scholar 

  19. Nakahata, T.; Ogawa, M.: Identification in culture of a new class of hemopoietic colony-forming units with extensive capability to self-renew and generate multipotential colonies. Proc. natn. Acad. Sci. USA79: 3843–3846 (1982).

    Article  CAS  Google Scholar 

  20. Williams, D.E.; Boswell, H.S.; Floyd, A.D.; Broxmeyer, H.E.: Pluripotential hematopoietic stem cells in post-5-fluorouracil murine bone marrow express the Thy-1 antigen. J. Immun.135: 1004–1011 (1985).

    PubMed  CAS  Google Scholar 

  21. Rowley, S.D.; Sharkis, S.J.; Hattenburg, C.; Sensenbrenner, L.: Culture from human bone marrow of blast progenitor cells with an extensive proliferative capacity. Blood69: 804–808 (1987).

    PubMed  CAS  Google Scholar 

  22. Leary, A.G.; Ogawa, M.: Blast cell colony assay for umbilical cord blood and adult bone marrow progenitors. Blood69: 953–956 (1987).

    PubMed  CAS  Google Scholar 

  23. Suda, T.; Suda, J.; Ogawa, M.: Proliferative kinetics and differentiation of murine blast cell colonies in culture: evidence for variable Go periods and constant doubling rates of early pluripotent hemopoietic progenitors. J. cell. Physiol.117: 308–318 (1983).

    Article  PubMed  CAS  Google Scholar 

  24. Suda, T.; Suda, J.; Ogawa, M.; Ihle, J.N.: Permissive role of interleukin-3 (IL-3) in proliferation and differentiation of multipotential hemopoietic progenitors in culture. J. cell. Physiol.124: 182–190 (1985).

    Article  PubMed  CAS  Google Scholar 

  25. Humphries, R.K.; Eaves, A.C.; Eaves, C.J.: Self-renewal of hemopoietic stem cell during mixed colony formation in vitro. Proc. natn. Acad. Sci. USA78: 3629–3633 (1981).

    Article  CAS  Google Scholar 

  26. Dresch, C.; Barreau, P.: Differentiation of mixed colony-forming cells in normal human bone marrow and blood. Exp. Hematol.13: 1143–1151 (1985).

    PubMed  CAS  Google Scholar 

  27. Broxmeyer, H.E.; Williams, D.E.; Cooper, S.; Shadduck, R.K.; Gillis, S.; Waheed, A.; Urdal, D.; Bicknell, D.C.: The comparative effects in vivo of recombinant murine interleukin-3, natural murine colony stimulating factor-1, and recombinant murine granulocyte-macrophage colony stimulating factor on myelopoiesis in mice. J. clin. Invest.79: 7210730 (1987).

    Article  Google Scholar 

  28. Hara, H.; Ogawa, M.: Murine hemopoietic colonies in culture containing normoblasts, macrophages and megakaryocytes. Am. J. Hematol.4: 23–34 (1978).

    Article  PubMed  CAS  Google Scholar 

  29. Messner, H.A.; Izaguirre, C.A.; Jamal, N.: Identification of T-lymphocytes in human mixed hemopoietic colonies. Blood58: 402–405 (1981).

    PubMed  CAS  Google Scholar 

  30. Maazen, R. van der; deWitte, T.; Blankenborg, G.; Wessels, J.; Hannen, C.: The origin of T-lymphocytes in human mixed hematopoietic colonies. Exp. Hematol.14: 624a (1986).

    Google Scholar 

  31. Bradley, T.R.; Hodgson, G.S.: Detection of primitive macrophages progenitor cells in mouse bone marrow. Blood54: 1446–1450 (1979).

    PubMed  CAS  Google Scholar 

  32. Boswell, H.S.; Wade, P.M.; Quesenberry, P.J.: Thy-1 antigen expression by murine high-proliferative capacity hematopoietic progenitor cells. J. Immun.133: 2940–2949 (1984).

    PubMed  CAS  Google Scholar 

  33. McNiece, I.; Kriegler, A.B.; Hapel, A.J.; Fung, M.C.; Young, I.G.; Bradley, T.R.; Hodgson, G.S.: Recombinant interleukin-3 exhibits synergistic factor activity. Cell Biol. int. Rep.8: 812 (1984).

    Article  PubMed  CAS  Google Scholar 

  34. Metcalf, D.: The molecular biology and functions of the granulocyte-macrophage colony-stimulating factors. Blood67: 257–267 (1986).

    PubMed  CAS  Google Scholar 

  35. Krantz, S.B.; Jacobsen, L.O.: Erythropoietin and the regulation of erythropoiesis. (University of Chicago Press, Chicago 1970).

    Google Scholar 

  36. Hoffman, R.; Yang, H.H.; Bruno, E.; Straneva, J.E.: Purification and partial characterization of a megakaryocyte colony-stimulating factor from human plasma. J. clin. Invest.75: 1174–1182 (1985).

    Article  PubMed  CAS  Google Scholar 

  37. Pluznik, D.H.; Sachs, L.: The cloning of normal ‘mast’ cells in tissue culture, J. cell. comp. Physiol.66: 319–324 (1966).

    Article  Google Scholar 

  38. Bradley, T.R.; Metcalf, D.: The growth of mouse bone marrow cells in vitro. Aust. J. exp. Biol. med. Sci.44: 287–300 (1966).

    Article  PubMed  CAS  Google Scholar 

  39. Metcalf, D.; Merchav, S.: Effects of GM-CSF deprivation on precursors of granulocytes and monocytes. J. cell. Physiol.112: 411–418 (1982).

    Article  PubMed  CAS  Google Scholar 

  40. Metcalf, D.; Merchav, S.; Wagemaker, G.: Commitment by GM-CSF or M-CSF of bipotential GM progenitor cells to granulocyte or macrophage formation; in Baum, Ledney, Thierfelder, Experimental hematology today (Karger, Basel 1982).

    Google Scholar 

  41. Metcalf, D.: Hemopoietic colonies (Springer, New York 1977).

    Google Scholar 

  42. Weisbart, R.H.; Golde, D.; Clark, S.C.; Wong, G.C.; Gasson, J.C.: Human granulocyte-macrophage colony-stimulating factor is a neutrophil activator. Nature, Lond.314: 361–363 (1985).

    Article  CAS  Google Scholar 

  43. Arnaout, M.A.; Wang, E.A.; Clark, S.C.; Sieff, C.A.: Human recombinant granulocyte-macrophage colony-stimulating factor increases cell-to-cell adhesion and surface expression of adhesion-promoting surface glycoproteins on mature granulocytes. J. clin. Invest.78: 597–601 (1986).

    Article  PubMed  CAS  Google Scholar 

  44. Silberstein, D.S.; Owen, W.F.; Gasson, J.C.; Di-Persio, J.F.; Golde, D.W.; Bing, J.C.; Soberman, R.; Austen, K.F.; David, J.R.: Enhancement of human eosinophil cytotoxicity and leukotriene synthesis by biosynthetic granulocyte-macrophage colony-stimulating factor. J. Immun.137: 3290–3294 (1986).

    PubMed  CAS  Google Scholar 

  45. Wing, E.J.; Magee, D.M.; Pearson, A.C.; Waheed, A.; Shadduck, R.K.: Peritoneal macrophages exposed to purified macrophage colony-stimulating factor (M-CSF) suppress mitogen- and antigen-stimulated lymphocyte proliferation. J. Immun.137: 2767–2773 (1986).

    Google Scholar 

  46. Fleischmann, J.; Golde, D.W.; Weisbart, R.H.; Gasson, J.C.: Granulocyte-macrophage colony-stimulating factor enhances phagocytosis of bacteria by human neutrophils. Blood68: 708–711 (1986).

    PubMed  CAS  Google Scholar 

  47. Grabstein, K.; Mochizuki, D.; Kronheim, s.; Price, V.; Cosman, D.; Urdal, D.; Gillis, S.; Conlon, P.: Regulation of antibody production in vitro by granulocyte-macrophage colony stimulating factor. J. mol. cell. Immunol.2: 199–207 (1986).

    PubMed  CAS  Google Scholar 

  48. Walker, F.; Nicola, N.A.; Metcalf, D.; Burgess, A.W.: Hierarchical down modulation of hemopoietic growth factor receptors. Cell43: 269–275 (1985).

    Article  PubMed  CAS  Google Scholar 

  49. Williams, D.E.; Chervinsky, D.S.; Orsini, F.; Tebbi, C.; Fitzpatrick, J.E.: In vitro production of granulocyte-macrophage colony-stimulating activity by murine bone marrow and spleen following Vinblastine administration in vivo. Hematol. Oncol.5: 35–42 (1987).

    Article  PubMed  CAS  Google Scholar 

  50. Iscove, N.N.; Guilbert, L.J.: Erythropoietin-independence of early erythropoiesis and a two-regulator model of proliferative control in the hemopoietic system; in Murphy, Peschle, Gordon, Mirand, In vitro aspects of erythropoiesis (Springer, New York 1978).

    Google Scholar 

  51. Iscove, N.N.: Erythropoietin-independent stimulation of early erythropoiesis in adult marrow cultures by conditioned medium from lectin-stimulated mouse spleen cells; in Golde, Cline, Metcalf, Fox, Hematopoietic cell differentiation (Academic Press, New York 1978).

    Google Scholar 

  52. Johnson, G.R.; Metcalf, D.: Pure and mixed erythroid colony formation in vitro stimulated by spleen cell conditioned medium with no detectable erythropoietin. Proc. natn. Acad. Sci. USA74: 3879–3883 (1977).

    Article  CAS  Google Scholar 

  53. Stephenson, J.R.; Axelrad, A.A.; McLeod, D.L.; Shreeve, M.M.: Induction of colonies of hemoglobin-synthesizing cells by erythropoietin in vitro. Proc. natn. Acad. Sci. USA68: 1542–1546 (1974).

    Article  Google Scholar 

  54. Tepperman, A.D.; Curtis, J.E.; McCulloch, E.A.: Erythropoietic colonies in cultures of human marrow. Blood44: 659–669 (1974).

    PubMed  CAS  Google Scholar 

  55. Long, M.W.; Gragawski, L.L.; Heffner, C.H.; Boxer, L.A.: Phorbol diesters stimulate the development of an early murine progenitor cell. The burst-forming unit megakaryocyte. J. clin. Invest.76: 431–438 (1985).

    Article  PubMed  CAS  Google Scholar 

  56. Metcalf, D.; MacDonald, H.R.; Odartchenko, N.; Sordat, B.: Growth of mouse megakaryocyte colonies in vitro. Proc. natn. Acad. Sci. USA72: 1744–1748 (1975).

    Article  CAS  Google Scholar 

  57. Quesenberry, P.J.; Ihle, J.N.; McGrath, E.: The effect of interleukin-3 and GM-CSA-2 on megakaryocyte and myeloid clonal colony formation. Blood65: 214–217 (1985).

    PubMed  CAS  Google Scholar 

  58. Yang, H.H.; Bruno, E.; Hoffman, R.: Studies of human megakaryocytopoiesis utilizing anti-megakaryocyte colony stimulating factor antibody. J. clin. Invest.77: 1873–1880 (1986).

    Article  PubMed  CAS  Google Scholar 

  59. Shapiro, H.M.: Practical flow cytometry (Liss, New York 1985).

    Google Scholar 

  60. Price, G.B.; Stewart, S.; Krogsrud, R.L.: Characterization of stem cell and progenitors of hemopoiesis by cell sorting. Blood Cells5: 161–174 (1979).

    PubMed  CAS  Google Scholar 

  61. Bodger, M.P.; Izaguirre, C.; Blacklock, H.A.; Hoffbrand, A.V.: Surface antigenic determinants on human pluripotent and unopotent hematopoietic progenitor cells. Blood61: 1006–1010 (1983).

    PubMed  CAS  Google Scholar 

  62. Andrews, R.G.; Torok-Storb, B.; Bernstein, I.D.: Myeloid-associated differentiation antigens on stem cells and their progeny identified by monoclonal antibodies. Blood62: 124–132 (1983).

    PubMed  CAS  Google Scholar 

  63. Ball, E.D.; Fanger, M.W.: The expression of myeloid-specific antigens on myeloid leukemia cells: correlation with leukemia subclasses and implications for normal myeloid differentiation. Blood61: 456–463 (1983).

    PubMed  CAS  Google Scholar 

  64. Civin, C.I.; Strauss, L.C.; Brovall, C.; Fackler, M.J.; Schwartz, J.F.; Shaper, J.H.: Antigenic analysis of hematopoiesis III. A hematopoietic progenitor cell surface antigen defined by a monoclonal raised against KG-1a cells. J. Immun.133: 157–165 (1984).

    PubMed  CAS  Google Scholar 

  65. Katz, F.E.; Tindle, R.; Sutherland, R.; Greaves, M.F.: Identification of a membrane glycoprotein associated with hematopoietic progenitor cells. Leuk. Res.9: 191–198 (1985).

    Article  PubMed  CAS  Google Scholar 

  66. Bacon, E.R.; Sytkowski, A.J.: Identification and characterization of a differentiation-specific antigen on normal and malignant murine erythroid cells. Blood69: 103–108 (1987).

    PubMed  CAS  Google Scholar 

  67. Goldschneider, I.; Metcalf, D.; Battye, F.; Mandel, T.: Analysis of rat hemopoietic cells on the fluorescence activated cell sorter. J. exp. Med.152: 419–437 (1980).

    Article  PubMed  CAS  Google Scholar 

  68. Visser, J.M.W.; Bauman, J.G.J.; Mulder, A.H.; Eliason, J.F.; deLeew, A.M.: Isolation of murine pluripotent hemopoietic stem cells. J. exp. Med.159: 1576–1590 (1984).

    Article  PubMed  CAS  Google Scholar 

  69. Lord, B.I.; Spooncer, E.: Isolation of hemopoietic spleen colony-forming cells. Lymphokine Res.5: 59–72 (1986).

    PubMed  CAS  Google Scholar 

  70. Nicola, N.A.; Metcalf D.; Melchner, H. von; Burgess, A.W.: Isolation of murine fetal hemopoietic progenitor cells and selective fractionation of various erythroid precursors. Blood58: 376–386 (1981).

    PubMed  CAS  Google Scholar 

  71. Lu, L.; Walker, D.; Broxmeyer, H.E.; Hoffman, R.; Hu, W.; Walker, E.: Characterization of adult human marrow hematopoietic progenitors highly enriched by two-color sorting with My10 and major histocompatibility (MHC) class II monoclonal antibodies. J. Immun. (in press).

  72. Falkenburg, J.H.F.; Fibbe, W.E.; Goselink, H.M.; vanRood, J.J.; Jansen, J.: Human hematopoietic progenitor cells in long-term, cultures express HLA-DR antigens and lack HLA-DQ antigens. J. exp. Med.162: 1359–1369 (1985).

    Article  PubMed  CAS  Google Scholar 

  73. Sieff, C.A.; Emerson, S.G.; Mufson, A.; Genser, T.A.; Nathan, D.G.: Dependence of highly enriched human bone marrow progenitors on hemopoietic growth factors and their response to recombinant erythropoietin. J. clin. Invest.77: 74–81 (1986).

    Article  PubMed  CAS  Google Scholar 

  74. Emerson, S.G.; Sieff, C.A.; Wang, E.A.; Wong, G.G.; Clark, S.C.; Nathan, D.G.: Purification of fetal hemopoietic progenitors and demonstration of recombinant multipotential colony-stimulating activity. J. clin. Invest.76: 1286–1290 (1985).

    Article  PubMed  CAS  Google Scholar 

  75. Griffin, J.D.; Beveridge, R.P.; Schlossman, S.F.: Isolation of myeloid progenitor cells from peripheral blood of chronic myelogenous leukemia patients. Blood60: 30–37 (1982).

    PubMed  CAS  Google Scholar 

  76. Koizumi, S.; Fine, R.L.; Curt, G.A.; Griffin, J.D.; Chabner, B.A.: Enrichment of myeloid progenitor cells from normal human bone marrow using an immune-rosette technique. Exp. Hematol.13: 560–565 (1985).

    PubMed  CAS  Google Scholar 

  77. Moore, M.A.S.; Broxmeyer, H.E.; Sheridan, A.P.C.; Jacobsen, N.; Winchester, R.J.: Continuous human bone marrow culture: Ia antigen characterization of probable pluripotential stem cells. Blood55: 682–690 (1980).

    PubMed  CAS  Google Scholar 

  78. Pretlow, T.G.; Pretlow, T.P.: Centrifugal elutriation (counterstreaming centrifugation) of cells. Cell. Biophys.4: 195–207 (1979).

    Article  Google Scholar 

  79. Metcalf, D.; MacDonald, H.R.: Heterogenity of in vitro colony and cluster-forming cells in the mose marrow: Segregation by velocity sedimentation. J. cell. Physiol.85: 643–654 (1975).

    Article  PubMed  CAS  Google Scholar 

  80. Nijhof, W.; Wierenga, P.K.: Isolation of hemopoietic pluripotent stem cells from thiamphenicol-pretreated mice. Expl Cell Res.155: 583–587 (1984).

    Article  CAS  Google Scholar 

  81. Williams, D.E.; Straneva, J.E.; Shen, R.N.; Broxmeyer, H.e.: Purification of murine bone marrow-derived granulocyte-macrophage colony-forming cells. Exp. Hematol.15: 243–250 (1987).

    PubMed  CAS  Google Scholar 

  82. Nijhof, W.; Wierenga, P.K.: Isolation and characterization of the erythroid progenitor cell: CFU-E. J. Cell Biol.96: 386–390 (1983).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Williams, D.E., Lu, L. & Broxmeyer, H.E. Characterization of hematopoietic stem and progenitor cells. Immunol Res 6, 294–304 (1987). https://doi.org/10.1007/BF02935524

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02935524

Keywords

Navigation