Abstract
Present methods and systems for the generation of human monoclonal antibodies are briefly reviewed. The specificities of the available reagents are outlined. It would appear that the generation using hybridoma methods of human monoclonal antibodies to human tumor cell surface antigens is a rare event and that methods ofin vitro immunostimulation may have to be used if such antibodies are to be reliably produced.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chiorazzi N, Wasserman R L, Kunkel H G: Use of Epstein-Barr virus-transformed B cell lines for the generation of immunoglobulin-producing human B cell hybridomas.J exp Med 156, 930 (1982).
Cote R J, Morrissey D M, Houghton A N, Beattie E J, Oettgen H F, Old L J: Generation of human monoclonal antibodies reactive with cellular antigens.Proc natn Acad sci 80, 2026 (1983).
Crawford D H, Barlow M J, Harrison J F, Winger L, Huehus E R: Production of human monoclonal antibody to rhesus D antigen.Lancet 1, 386 (1983).
Croce C M, Linnenbach A, Hall W, Steplewski Z, Koprowski H: Production of human hybridomas secreting antibody to measles virus.Nature 288, 488 (1980).
Edwards P A W, Smith C M, Neville A M, O'Hare M J: A human-human hybridoma system based on a fast-growing mutant of the ARH-77 plasma cell leukemia-derived line.Eur J Immun 12, 641 (1982).
Eisenbarth G S, Linnenbach A, Jackson R, Scearce R, Croce C M: Human hybridomas secreting anti-islet auto antibodies.Nature 300, 264 (1982).
Handley H H, Royston I: A human lymphoblastoid B cell line useful for generating immunoglobulin secreting human hybridomas, in Mitchell M S and Oettgen H F (eds):Hybridomas in Cancer Diagnosis and Treatment, p. 125. New York, Raven Press (1982).
Houghton A N, Brooks H, Cote R J, Taormina M C, Oettgen H F, Old L J: Detection of cell surface and intracellular antigens by human monoclonal antibodies: Hybrid cell lines derived from lymphocytes of patients with malignant melanoma.J exp Med 158, 53 (1983).
Köhler G, Milstein C: Derivation of specific antibodyproducing tissue culture and tumor lines by cell fusion.Eur J Immun 6, 511 (1976).
Kozbor D, Lagarde A E, Roder J C: Human hybridomas constructed with antigen-specific Epstein-Barr virus-transformed cell lines.Proc natn Acad Sci 79, 6651 (1982).
Kozbor D, Roder J C: The production of monoclonal antibodies from human lymphocytes.Immun Today 4, 72 (1983).
Lane H C, Shelhamer J H, Mostowski H S, Fauci A S: Human monoclonal anti-keyhole limpet hemocyanin antibody-secreting hybridoma produced from peripheral blood B lymphocytes of a keyhole limpet hemocyanin-immune individual.J exp Med 155, 333 (1982).
Neville A M, Foster C S, Moshakis V, Gore M: Monoclonal antibodies and human tumor pathology.Hum Pathol 13, 1067 (1982).
Nowinski R, Berglund C, Lane J, Lostrom M, Bernstein I, Young W, Hakomori S-I, Hill L, Cooney M: Human monoclonal antibody against Forsmann antigen.Science 210, 537 (1980).
O'Hare M J: Monoclonal antibodies of murine and human origin: their generation, characterization and use, in Panayi G S, David C. (eds):International Medical Reviews: Clinical Immunogenetics. Butterworths (in press).
Old L J: The search for specificity—GHA Clowes Memorial lecture.Cancer Res 41, 361 (1981).
Olsson L, Kaplan H S: Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity.Proc natn Acad Sci 77, 5429 (1980).
Philips J, Sikora K, Watson J V: Localisation of glioma by human monoclonal antibody.Lancet 2, 1215 (1982).
Ritts R E, Ruiz-Arguelles A, Weyl K G, Bradley A L, Weihmeir B, Jacobsen D J, Strehlo B L: Establishment and characterization of a human nonsecretory plasmacytoid cell line and its hybridization with human B cells.Int J Cancer 31, 133 (1983).
Schlom J, Wunderlich D, Teramoto Y A: Generation of human monoclonal antibodies reactive with human mammary carcinoma cells.Proc natn Acad Sci 77, 6841 (1980).
Sikora K, Alderson T, Ellis J, Watson J: Human hybridomas from patients with malignant disease.Br J Cancer 47, 135 (1983).
Steinitz M, Klein G, Koskimies S, Makel O: EB virusinduced B lymphocyte cell lines producing specific antibody.Nature 269, 420 (1977).
Steinitz M, Tamir S: Human monoclonal autoimmune antibody producedin vitro: rheumatoid factor generated by Epstein-Barr virus transformed cell line.Eur J Immunol 12, 126 (1982).
Valente W A, Vitti P, Yavin Z, Yavin E, Rotella C M, Grollman E F: Monoclonal antibodies to the thyrotropin receptor: stimulating and blocking antibodies derived from the lymphocytes of patients with Graves' disease.Proc natn Acad Sci 79, 6680 (1982).
Wunderlich D, Teramoto Y A, Alford C, Schlom J: The use of lymphocytes from axillary lymph nodes of mastectomy patients to generate human monoclonal antibodies.Eur J Cancer clin Oncol 17, 719 (1981).
Zurawski V R, Haber E, Black P H: Production of antibody to tetanus toxoid by continuous human lymphoblastoid cell lines.Science 199, 1439 (1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Neville, A.M., Edwards, P.A.W. & O'Hare, M.J. Generating human monoclonal antibodies. Med. Oncol. & Tumor Pharmacother. 1, 73–76 (1984). https://doi.org/10.1007/BF02934977
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02934977