Abstract
Objective: To explore the effects of Schisanhenol (Sal) on generation of reactive oxygen species (ROS) including superoxide anion, hydrogen peroxide and hydroxyl radical as well as on the activity of superoxide dismutase (SOD) and catalase in respiratory burst of rat neutrophils.Methods: Rat neutrophils were prepared from male Wistar rats. Superoxide anion, hydrogen peroxide, hydroxyl radical, SOD and catalase activity were measured.Results: Sal (1, 10, 100 μmol/L) inhibited generation of ROS of rat neutrophils during respiratory burst induced by formyl-methionyleucylphenylalanine (FMLP) and phorbol myristate acetate (PMA) dosedependently, and it enhanced SOD and catalase activities.Conclusion: Sal may inhibit generation of ROS and enhance activity of anti-oxidant enzymes of neutrophils to reduce oxidative injury to tissues.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Liu GT. Hepato-pharmacology of Fructus schizandrea. In Advances in Chinese Medicinal Materials Research. (Chang HM et al Eds) World Scientific Publ. Co., Singapore, 1985: 257.
Liu GT, Wei H. Induction of hepatic microsomal monooxygenase by schisanhenol in rats. Acta Pharmacol Sin 1985; 6: 41.
MacDonald JR, Bechstead JH, Smuckler EA. An ultrastructural and histochemical study of the prominent inflammatory response in D(+)-galactosamine hepatotoxicity. Br J Exp Pathol 1987 68: 189.
Schlayer HJ, Laaff H, Peters P, et al: Involvement of tumor necrosis factor in endotoxin-triggered neutrophil adherence to sinusoidal endothelial cells of mouse liver and its modulation in acute phase. J Hepatol 1988; 7: 239.
Stossel T. Evaluation of opsonic and leukocyte function with a spectrophotometric test in patients with infection and with phagocytic disorders. Blood 1973; 42: 121.
Nathan CF, Brukner LH, Silverstein SC, et al. Extracellular cytolysis by activated macrophages and granulocytes. I. Pharmacologic triggering of effector cells and the release of hydrogen peroxide. J Exp Med 1979; 149: 84.
Onodra T and Ashraf M. Detection of hydroxyl radicals in the post-ischemic reperfused heart using salicylate as a trapping agent. J Mol Cell Cardial 1991; 23: 365.
Beauchamp C, Fridovich I. Superoxide dismutase: applicable improved assays and an assay to acrylamide gels. Anal Biochem 1971; 44: 276.
Nelson DP, Kiesow LA. Ethalpy of decomposition of hydrogen peroxide by catalase 25 C (with molar extinction coefficients of H2O2 solution in the UV). Anal Biochem 1972; 49: 474.
Klebanoff SJ. Oxygen metabolism and the toxic properties of phagocytes. Ann Inter Med 1980; 93: 480.
Ward PA, Till GO, Kundel R, et al. Evidence for role of hydroxyl radical in complement and neutrophil-dependent tissue injury. J Clin Invest 1983; 72: 789.
Lu H, Liu GT. Effects of nine dibenzo (a, c) cyclooctene ligands isolated from Fructus Schizandrae on lipid peroxidation and antioxidative enzyme activity. Chem-Biol Interactions 1991; 78: 77.
Lu H, Liu GT. Anti-oxidant activity of dibenzocyclooctene lignands isolated from schisandraceae. Planta Medica 1992; 58: 311.
Zhang KJ, Lin TJ, Liu GT. Effect of schisanhenol on function and surface shape of rat neutrophils. Acta Pharmacol Sin 1995; 16: 234.
Lin TJ, Liu GT, Li XJ, et al. Detection of free radical scavenging activity of schisanhenol by electron spin resonance. Acta Pharmacol Sin 1990; 11: 5.
Author information
Authors and Affiliations
Additional information
Project was supported by the National Science Foundation of China (No. 39170232) and Huo Yingdong Education Foundation
Rights and permissions
About this article
Cite this article
Kejian, Z., Gengtao, L. Effects of Schisanhenol on respiratory burst in rat neutrophils. CJIM 5, 134–137 (1999). https://doi.org/10.1007/BF02934666
Issue Date:
DOI: https://doi.org/10.1007/BF02934666