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Trophic effects of glicentin on rat small-intestinal mucosa in vivo and in vitro

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Abstract

To define the role of glicentin the active site of enteroglucagon, we evaluated the trophic effects of recombinant rat glicentin on rat small intestine and IEC-6 cells. In vivo, a significant increase was observed in jejunal wet weight, protein content, DNA content, and alkaline phosphatase activity after the subcutaneous administration of 100 μg/kg per day of glicentin for 2 weeks. In the ileum, however, there were no significant differences between the control versus glicentin groups in any of these parameters. Ornithine decarboxylase (ODC) activity 3.5 h after an intraperitoneal injection of glicentin was increased in the jejunal mucosa, but not in the ileal mucosa. In vitro, glicentin, at a dose of more than 100 ng/ml, significantly increased both tritium-thymidine incorporation and the number of IEC-6 cells. These findings indicate that glicentin exerts direct trophic effects on the rat small-intestinal mucosa and on the rat small-intestinal cell line, IEC-6, and that this peptide appears to be an active site of enteroglucagon.

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Authors and Affiliations

  1. Second Department of Internal Medicine, Shiga University of Medical Science, Tsukinowa, Seta, 520-21, Otsu, Shiga, Japan

    Satoshi Myojo, Tomoyuki Tsujikawa, Masaya Sasaki, Yoshihide Fujiyama & Tadao Bamba

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  1. Satoshi Myojo
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  2. Tomoyuki Tsujikawa
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  3. Masaya Sasaki
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  4. Yoshihide Fujiyama
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  5. Tadao Bamba
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Myojo, S., Tsujikawa, T., Sasaki, M. et al. Trophic effects of glicentin on rat small-intestinal mucosa in vivo and in vitro. J Gastroenterol 32, 300–305 (1997). https://doi.org/10.1007/BF02934484

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  • DOI: https://doi.org/10.1007/BF02934484

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