Skip to main content
SpringerLink
Log in

Analysis of variations in RAPD profiles among accessions ofProsopis

  • Published:
Journal of Genetics Aims and scope Submit manuscript

Abstract

The genetic variability among accessions ofProsopis was determined using randomly amplified polymorphic DNA (RAPD) profiles. Similarities of profiles were determined using the algorithm of Jaccard, and UPGMA and neighbour joining trees were generated from the similarity data. The average similarity was highest among the accessions ofP. glandulosa (0.52 ± 0.18) and least in the accessions ofP. juliflora (0.37 ± 0.15), indicating that the latter species has greater diversity among accessions. Our observations suggest that RAPD analysis could help in identifying genetic variations among different accessions ofProsopis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adams R. P. and Demeke T. 1993 Systematic relationship inJuniperus based on random amplified polymorphic DNAs (RAPD).Taxon 42, 553–571.

    Article  Google Scholar 

  • Armstrong J. S., Gibbs A. J., Peakall R. and Weiler G. 1994 The RAPDistance package, ver. 1.03. Research School of Biological Sciences, Australian National University, Canberra, Australia.

    Google Scholar 

  • Arya S., Bisht R. P., Tomar R., Toky O. P. and Harris P. J. C. 1995 Genetic variation in minerals, crude protein and structural carbohydrates of foliage in provenances of young plants ofProsopis cineraria (L.) Druce in India.Agrofor. Syst. 29, 1–7.

    Article  Google Scholar 

  • Bradshaw H. D., Villar M., Watson B. D., Otto K. G., Stewart S. and Stettler R. R 1994 Molecular genetics of growth and development inPopulus. III. A genetic linkage map of a hybrid poplar composed of RFLP, STS and RAPD markers.Theor. Appl. Genet. 89, 167–178.

    CAS  Google Scholar 

  • Burkart A. 1976 A monograph of the genusProsopis (Leguminosae, Subfamily Mimosoideae).j. Arnold Arbor. 57, 219–249, 450–524.

    Google Scholar 

  • Carman N. J., Dossaji S. R and Mabry T. J. 1974 A populational survey of amino acids inProsopis species from North and South America.Biochem. Syst. Ecol. 2, 73–74.

    Article  Google Scholar 

  • Chalmers K. J., Newton A. C, Waugh R., Wilson J. and Powell W. 1994 Rvaluation of the extent of genetic variation in mahoganies using RAPD markers.Theor. Appl. Genet. 89, 504–508.

    Article  Google Scholar 

  • Corniquel B. and Mercier L. 1994 Date palm (Phoenix dactylifera L.) cultivar identification by RFLP and RAPD.Plant Sci. 101, 163–172.

    Article  CAS  Google Scholar 

  • Hunziker J. H., Saidman B. O., Naranjo C. A., Palacois R. A., Poggio L. and Burghardt A. D. 1986 Hybridization and genetic variation of Argentine species ofProsopis.For. Ecol. Manage. 16, 301–315.

    Article  Google Scholar 

  • Jaccard P. 1901 Rtude comparative de la distribution florale dans une portion des Alpes et des Jura.Bull. Soc. Vaudoise Sci. Nat. 37, 547–579.

    Google Scholar 

  • de Kochko A. and Hamon S. 1990 A rapid and efficient method for the isolation of restrictable DNA from plants of the genusAbelmoschus.Plant Mol. Biol. Rep. 8, 3–7.

    Article  Google Scholar 

  • Lamarque A. L., Maestri D. M., Grosso N. R., Zygadlo J. A. and Guzman C. A. 1994 Proximate composition and seed lipid components of someProsopis (Leguminosae) from Argentina.J. Sci. Food Agric. 66, 323–326.

    Article  CAS  Google Scholar 

  • Leakey R. B. and Last R T. 1980 Biology and potential ofProsopis species in arid environment with particular reference toP. cineraria, j. Arid Environ. 3, 9–24.

    Google Scholar 

  • Naranjo C. A., Poggio L. and Zeiger S. E. 1984 Phenol chromatography, morphology and cytogenetics in three species and natural hybrids ofProsopis (Leguminosae, Mimisoideae).Plant Syst. Evol. 144, 257–276.

    Article  CAS  Google Scholar 

  • Ranade S. A., Kumar A., Goswami M., Farooqui N. and Sane P. V. 1997 Genome analysis of amaranths: determination of inter- and intra-species variations.J. Biosci. 22, 451–464.

    Article  Google Scholar 

  • Saidman B. O. and Vilardi J. C. 1987 Analysis of genetic similarities among seven species ofProsopis (Leguminosae: Mimosoideae).Theor. Appl. Genet. 75, 109–116.

    Article  Google Scholar 

  • Saidman B. O. and Vilardi J. C. 1993 Genetic variability and germplasm conservation in the genusProsopis. InNursery technology for agroforestry. Applications in arid and semiarid regions (ed. Sunil Puri and P. K. Khosla), pp. 187–198. Oxford and IBH, New Delhi.

    Google Scholar 

  • Saitou N. and Nei M. 1987 The neighbor-joining method: a new method for reconstructing phylogenetic trees.Mol. Biol. Evol. 4, 406–25.

    PubMed  CAS  Google Scholar 

  • Sambrook J., Fritsch E. F. and Maniatis T. 1989Molecular cloning. A laboratory course manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.

    Google Scholar 

  • Schinini A. 1981 Contribucion a la flora del Paraguay.Bonplandia 5, 101–108.

    Google Scholar 

  • Sokal R. R. and Sneath P. H. A. 1963Principles of numerical taxonomy. Freeman, San Francisco.

    Google Scholar 

  • Stiles J. I., Lemme C, Sondur S., Morshidi M. B. and Manshardt R. 1993 Using randomly amplified polymorphic DNA for evaluating genetic relationships among papaya cultivars.Theor. Appl. Genet. 85, 697–701.

    Article  CAS  Google Scholar 

  • Williams J. G. K., Kubelik A. R., Livak K. J., Rafalski J. A. and Tingey S. V. 1990 DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.Nucl. Acids Res. 18, 6531–6535.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant Molecular Biology Division, International Centre for Genetic Engineering and Biotechnology, 110067, New Delhi, India

    Mamta Goswami

  2. Plant Molecular Biology Division, National Botanical Research Institute, Rana Pratap Marg, 226001, Lucknow, India

    Shirish Anand Ranade

Authors
  1. Mamta Goswami
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shirish Anand Ranade
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shirish Anand Ranade.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Goswami, M., Anand Ranade, S. Analysis of variations in RAPD profiles among accessions ofProsopis . J. Genet. 78, 141–147 (1999). https://doi.org/10.1007/BF02934459

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02934459

Keywords

Search

Navigation