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Kinetic model for biotransformation of digitoxin in plant cell suspension culture ofDigitalis lanata

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Abstract

Batch suspension cultures ofDigitalis lanata plant cell were performed to investigate the biotransformation of digitoxin.Digitalis lanata K3OHD plant cells were used to biotransform digitoxin into deacetyllanatoside C. A kinetic model was proposed to describe cell growth, substrate consumption, depletion of digitoxin, formation and depletion of digoxin and purpureaglycoside A, and formation of deacetyllanatoside C. The digoxin and purpureaglycoside A are intermediates of deacetyllanatoside C formation from digitoxin. Interactions between extracellular and intracellular compounds were considered. The proposed model could accurately predict cell growth, substrate consumption and product synthesis. And it can provide a useful framework for quantitative analysis of biotransformation in a plant cell culture system.

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References

  1. Kutney, J. P. (1998) Biotechnology and synthetic chemistry routes to clinically important compounds.Pure Appl. Chem. 70: 2093–2100.

    Article  CAS  Google Scholar 

  2. Krings, U., and R. G. Berger (1998) Biotechnological production of flavours and fragrances.Appl. Microbiol. Biotechnol. 49: 1–8.

    Article  CAS  Google Scholar 

  3. Prenosil, J. E., and H. Pedersen (1983) Immobilized plant cell reactors.Enzyme Microb Technol. 5: 323–331.

    Article  CAS  Google Scholar 

  4. Panda, A. K., S. Mishira, V. S. Bisaria, and S. S. Bhojwani, (1989) Plant cell reactors—a perspective.Enzyme Microb. Technol. 11: 386–397.

    Article  CAS  Google Scholar 

  5. Buitelaar, R. M., and J. M. Tramper (1992) Strategies to improve the production of secondary metabolites with plant cell culture; a literature review.J. Biotechnol. 23: 111–141.

    Article  CAS  Google Scholar 

  6. Kutney, J. P. (1997) Plant cell culture combined with chemistry-routes to clinically important compounds.Pure Appl. Chem. 69: 431–436.

    Article  CAS  Google Scholar 

  7. Kreis, W., and E. Reinhard (1992) 12β-Hydroxylation of digitoxin by suspension-culturedDigitalis lanata cells: Production of digoxin in 20-litre and 300-litre air-lift bioreactors.J. Biotechnol. 26: 257–273.

    Article  CAS  Google Scholar 

  8. Kreis, W., and E. Reinhard (1988) 12β-Hydroxylation of digitoxin by suspension-culturedDigitalis lanata cells: Production of deacetyllantoside C using a two-stage culture method.Planta Med. 54: 143–148.

    Article  CAS  Google Scholar 

  9. Kreis, W., W. Zhu, and E. Reinhard (1989) 12β-Hydroxylation of digitoxin by suspension-culturedDigitalis lanata cells: Production of deacetyllantoside C in a 20-litre air-lift bioreactors.Biotechnol. Lett. 11: 325–330.

    Article  CAS  Google Scholar 

  10. Lee, J.-E., S.-Y. Lee, and D.-I. Kim (1999) Increased production of digoxin by digitoxin biotransformation using cyclodextrin polymer inDigitalis lanata cell cultures.Biotechnol. Bioprocess Eng. 4: 32–35.

    Article  CAS  Google Scholar 

  11. Bailey, J. E., and D. F. Ollis (1986)Biochemical Engineering Fundamentals. p. 265–269, 2nd ed. McGraw-Hill Inc. New York, NY, U.S.A.

    Google Scholar 

  12. Mulchandani, A. and J. H. T. Luong (1989) Microbial kinetics revisited.Enzyme Microb. Technol. 11: 66–73.

    Article  CAS  Google Scholar 

  13. Metzler, C. M., G. L. Elfring, and A. J. McEwen (1974) A package of computer programs for pharmaco-kinetic modeling.Biometrics 30: 562–563.

    Article  Google Scholar 

  14. Seinfeld, J., and L. Lapidus (1970)Process Modeling Estimation and Identification. Prentice-Hall Inc. Englewood Cliffs, NJ, U.S.A.

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Engineering, Sogang University, C.P.O. Box 1142, 100-611, Seoul, Korea

    Jeong-Woo Choi, Young-Kee Kim, Hyun-Kyu Park & Won Hong Lee

  2. Department of Biological Engineering, Inha University, 402-751, Inchon, Korea

    Dong-Il Kim

Authors
  1. Jeong-Woo Choi
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  2. Young-Kee Kim
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  3. Hyun-Kyu Park
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  4. Won Hong Lee
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  5. Dong-Il Kim
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Correspondence to Jeong-Woo Choi.

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Choi, JW., Kim, YK., Park, HK. et al. Kinetic model for biotransformation of digitoxin in plant cell suspension culture ofDigitalis lanata . Biotechnol. Bioprocess Eng. 4, 281–286 (1999). https://doi.org/10.1007/BF02933754

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  • DOI: https://doi.org/10.1007/BF02933754

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