Abstract
Two-phase cultures ofTaxus cuspidata were performed using silicone cubes as a second phase in shake flasks for paclitaxel production. Among various taxanes, paclitaxel was selectively adsorbed on the silicon cubes. When silicone cubes were added to suspension culture ofTaxus cuspidata, paclitaxel production increased about 45 folds. The maximum paclitaxel production was 3.95 mg/L when 10% of silicone cubes were added to the culture at the 7th day from inoculation.
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Slichenmyer, W. J. and D. D. Von Hoff (1991) Taxol: a new and effective anti-cancer drug.Anti-cancer Drugs 2: 519–530.
Joyce, C. (1993) Taxol: Search for Cancer Drug.Bio Science 43(3): 133–136.
Stull, D. P., T. A. Scales, R. Daughenbaugh, N. A. Jans, and D. T. Bailey (1995) Taxol (Paclitaxel): Strategies to increase the supply of a new anticancer drug.Appl. Biochem. Biotechnol. 54: 133–140.
Jaziri, M., A. Zhiri, Y. W. Guo, J. P. Dupont, K. Shimomura, H. Hamada, M. Vanhaelen, and J. Homès (1996)Taxus sp. cell, tissue and organ cultures as alternative sources for taxoids production: a literature survey.Plant Cell Tiss. Org. Cult. 46: 59–75.
Ketchum, R. E. B., D. M. Gibson, R. B. Croteau, and M. L. Shuler (1999) The kinetics of taxoid accumulation in cell suspension cultures ofTaxus following elicitation with methyl jasmonate.Biotechnol. Bioeng. 62: 97–105.
Pestchanker, L. J., S. C. Roberts, and M. L. Shuler (1996) Kinetics of taxol production and nutrient use in suspension culture ofTaxus cuspidata in shake flask and wilson-type bioreactor.Enzyme Microb. Technol. 19: 256–260.
Hezari, M., R. E. B. Ketchum, D. M. Gibson and R. Croteau (1997) Taxol production and taxadiene synthase activity inTaxus canadenis cell suspension cultures.Arch. Biochem. Biophys. 337: 185–190.
Fett-Neto, A. G., W. Y. Zhang, and F. DiCosmo (1994) Kinetics of taxol production, growth, and nutrient uptake in cell suspensions ofTaxus cuspidata.Biotechnol. Bioeng. 44: 205–210.
Ciddi, V., V. Srinivasan, and M. L., Shuler (1995) Elicitation ofTaxus up. cell cultures for production of taxol.Biotechnol. Lett. 17: 1343–1346.
Moon, W. J., B. S. Yoo, D.-I. Kim, and S. Y. Byun (1998) Ehciation kinetics of taxane production in suspension cultures ofTaxus baccata Pendular.Biotechnol. Tech. 12: 79–81.
Kwon, I. C., Y. J. Yoo, J. H. Lee, and J. O. Hyun (1998) Enhancement of taxol production byin situ recovery of product.Process Biochem. 33: 701–707.
Park, J. H., C. H. Kim, S. W. Lee, and I. S. Chung (1995) Production of taxol fromin vitro cell culture ofTaxus cuspidata.Acta Horticulturae 390: 127–132.
Beiderbeck, R. and B. Knoop (1987) Two phase culture. p. 255–266 In: Vasil, I. K. (ed.)Cell Culture and Somatic Cell Genetics of Plants. Vol 4, Academic Press. San Diego, U.S.A.
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Kim, CH., Hong, SH., Son, SH. et al. Improved paclitaxel production in a two-phase suspension culture ofTaxus cuspidata using silicone cubes. Biotechnol. Bioprocess Eng. 4, 273–276 (1999). https://doi.org/10.1007/BF02933752
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DOI: https://doi.org/10.1007/BF02933752