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Morphogenic studies in tissue cultures of the parasiteSantalum album L.

Morfogenetické studie explantátových kultur parasitické rostlinySantalum album L.

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Biologia Plantarum


Whole seeds, excised embryos, and excised endosperm ofSantalum album were aseptically cultured with a view to studying seed germination in isolation from the host species, and to establishing callus cultures from both embryo and endosperm for comparative studies et their morphogenesis. Seed germination and seedling formation occurred normally only on modified White's medium supplemented with casein hydrolysate or coconut milk, or with both substances. Neither the excised embryo nor the endosperm grew on any of the culture media tested. However in about 17 per cent seed cultures on White's medium supplemented with 2,4-D, kinetin, and yeast extract, the endosperm degenerated, whereas the embryo callused and subsequently differentiated into innumerable embryoids; eventually the embryoids developed into normal plantlets. Callusing of the endosperm occurred also in seed cultures on four media supplemented variously with 2,4-D, kinetin, and yeast extract. Although the endosperm tissue grew through several passages no organ fornation was observed.


Celistvá semena, extirpovaná embrya a extirpovaný endosperm rostlinSantalum album byly kultivovány za aseptických podmínek. Účelem práce bylo studovat klíčení semen v nepřítomnosti hostitelské rostliny a získat kalusové kultury z embryí i endospermu k srovnávacím morfogenetickým pokusům. Klíčení semen a utváření klíčních rostlin probíhalo normálně pouze na Whiteově mediu, k němuž byl dodán kasein-hydrolysát nebo kokosové mléko, anebo obě tyto látky. Extirpovaná embrya ani endosperm nerostly na žádném z testovaných kultivačních medií. V 17 % semen kultivovaných na Whiteově mediu, doplněném 2,4-D, kinetinem a kvasničným extraktem, docházelo k degeneraci endospermu, kdežto z embrya se vytvořil kalus, který postupně diferencoval ve velmi četné embryoidy; z embryoidů se nakonec vyvinuly normální rostlinky. K tvorbě kalusů z endospermu došlo také v semenech kultivovaných na čtyřoch půdách, k nimž byly přidány 2,4-D, kinetin a kvasničný extrakt v různých kombinacích. Ačkoliv pletivo z endospermu rostlo po několik pasáží, nebyla pozorována tvorba orgánů.

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Rao, P.S., Rangaswamy, N.S. Morphogenic studies in tissue cultures of the parasiteSantalum album L.. Biol Plant 13, 200–206 (1971).

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