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Autoradiography of tritiated uridine in shoot apices ofChenopodium rubrum

Autoradiografie uridinu-3H u osních vrcholůChenopodium rubrum

  • Published:
Biologia Plantarum

Abstract

The incorporation of uridine-5-3H into shoot apices ofChenopodium rubrum, plants was studied using autoradiography. The evaluation of the rate of incorporation into the nucleolus and the extranucleolar part of the nucleus as a function of the total radioactivity in the apex yields quantitative data on the distribution of labeling in these parts of the nucleus. Incubation of intact germinating plants in uridine-3H makes it possible to carry out chase experiments.

Curves of uridine incorporation into the nucleolus and the extranucleolar part of the nucleus were obtained which demonstrated a non-linear course of incorporation. When incubating with uridine from 30 to 120 min the nucleolar/extranucleolar ratio of labelling was found to increase from 2 to 3. In chase experiments this ratio changed within three days from 3 to 1.

Interpretation of these results in view of the function of RNA localized in different parts of the nucleus is discussed.

Abstract

Inkorporace uridinu-5-3H do osních vrcholů rostlinekChenopodium rubrum byla sledována v autoradiogramech. Hodnocení rychlosti inkorporace do jadérka a mimojadérkové části jádra v závislosti na celkové radioaktivitě ve vrcholu postkytuje kvantitativní údage o distribuci značení v těchto složkách jádra. Inkubace intaktních klíčních rostlin v uridinu-3H umožňuje provedení “chase” pokusů.

Byly získány křivky inkorporace do jadérka a mimojadérkové části rostlin, které ukázaly, že při stoupající celkové radioaktivitě je průběh inkorporace do těchto složek nelineární. Při inkubaci v uridinu od 30 do 120 minut stoupal poměr značení ve prospěch jadérka z 2∶1 na 3∶1. V “chase” pokusech se tento poměr během tří dnů měnil z 3∶1 na 1∶1.

Jsou diskutovány možnosti interpretace těchto výsledků z hlediska funkce různě lokalizované RNA.

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Authors and Affiliations

  1. Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha

    Frideta Seidlová

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  1. Frideta Seidlová
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Address: Ke dvoru 15, Praha — Vokovice, Czechoslovakia.

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Seidlová, F. Autoradiography of tritiated uridine in shoot apices ofChenopodium rubrum . Biol Plant 13, 189–192 (1971). https://doi.org/10.1007/BF02933635

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  • DOI: https://doi.org/10.1007/BF02933635

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