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Effect of aeration on denitrification byOchrobactrum anthropi SY509

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Abstract

Aeration was found to affect the biological denitrification byOchrobactrum anthropi SY509. Although cell growth was vigorous under 1 vvm of aeration and an agitation speed of 400 rpm in a 3-L jar fermentor, almost no nitrate was removed. Yet under low agitation speeds (100, 200, and 300 rpm), denitrification occurred when the dissolved oxygen was exhausted shortly after the inoculation of the microorganism.Ochrobactrum anthropi SY509 was found to express highly active denitrifying enzymes under anaerobic conditions. The microorganism also synthesized denitrifying enzymes under aerobic conditions (1 vvm and 400 rpm), yet their activity was only 60% of the maximum level under anaerobic conditions and the nitrate removal efficiency was merely 15%. However, although the activities of the denitrifying enzymes were inhibited in the presence of oxygen, they were fully recovered when the conditions were switched to anaerobic conditions.

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References

  1. Keith, L. H. and W. A. Telliard (1979) Priority pollutants: 1. A perspective view.Environ. Sci. Technol. 13: 416–423.

    Article  Google Scholar 

  2. World Health Organization (1984) Guidelines for drinking water quality. Geneva, Switzerland.

  3. National Acacdemy of Science (1978) Nitrates: An environmental assessment, Washington, DC, USA.

  4. Mirvish, S. S. (1977) N-nitroso compounds, nitrate, and nitrite: possible implications for the causation of human cancer.Prog. Water Technol. 8: 16.

    Google Scholar 

  5. Knowles, R. (1982) Denitrification.Microbiol. Rev. 46: 43–70.

    CAS  Google Scholar 

  6. Hochstein, L. I. and G. A. Tomlinson (1988) The enzymes associated with denitrification.Annu. Rev. Microbiol. 42: 231–261.

    Article  CAS  Google Scholar 

  7. Sung, D. W., S. H. Song, J. H. Kim, and Y. J. Yoo (2002) Effects of electron donors on nitrate removal by nitrate and nitrite reductases.Biotechnol. Bioprocess Eng. 7: 112–116.

    Article  CAS  Google Scholar 

  8. Park, E. J., J. K. Seo, J. K. Kim, K. H. Suh, and S. K. Kim (2000) Denitrification characteristics and microorganism composition of acclimated denitrification consortium.J. Microbiol. Biotechnol. 10: 410–414.

    CAS  Google Scholar 

  9. Tirdje, J. M. (1988) Ecology of denitrification and dissimilatory nitrate reduction to ammonia. In:Biology of Anaerobic Microorganisms. Zehnder, A. J. B. (ed.), pp. 179–244. Wiley, New York, USA.

    Google Scholar 

  10. Alefounder, P. R., A. J. Gueenfield, J. E. G. McCarthy, and S. J. Ferguson (1983) Selection and organization of denitrifying electron-transfer pathway inParacoccus denitrificans.Biochim. Biophys. Acta. 724: 20–39.

    Article  CAS  Google Scholar 

  11. Loyd D., L. Boddy, and K. J. P. Davies (1987) Persistence of bacterial denitrification capacity under aerobic conditions: the rule rather than the exception.FEMS Microb. Ecol. 45: 185–190.

    Article  Google Scholar 

  12. Blaszczyk, M. (1993) Effect of medium composition on the denitrification of nitrate by paracoccus denitrifications.Appl. Environ. Microbiol. 59: 3951–3953.

    CAS  Google Scholar 

  13. Almeida, J. S., M. A. M. Reis, and M. J. T. Carrondo (1995) Competition between nitrate and nitrite reduction in denitrification byPseudomonas fluorescens.Biotechnol. Bioeng. 46: 476–484.

    Article  CAS  Google Scholar 

  14. Hwang, S. H., Y. H. Lee, and M. H. Cho (1999) Isolation and characteristics of denitrifying Pseudomonas CW4.Kor. J. Biotechnol. Bioeng. 14: 616–620.

    Google Scholar 

  15. Chauret, C. and R. Knowles (1991) Effect of tungsten on nitrate and nitrite reductase inAzospirillum brasilense Sp7.Can. J. Microbiol. 37: 744–750.

    Article  CAS  Google Scholar 

  16. McGregor, C. H. (1978) Isolation and characterization of nitrate reductase fromEscherichia coli.Methods Enzymol. 53: 347–355.

    Article  Google Scholar 

  17. Zumft, W. G. (1997) Cell biology and molecular basis of denitrification.Microbiol. Molecular Biol. Rev. 61: 533–616.

    CAS  Google Scholar 

  18. Hewitt, L. F. (1950),Oxidation-reduction Potentials, in Bacteriology and Biochemistry. 6th ed., Livingstone, Edinburgh.

    Google Scholar 

  19. Bell, L. C., D. J. Richardson, and S. J. Ferguson (1990) Periplasmic and membrane-bound respiratory nitrate reductases inThiosphaera pantotropha.FEBS Lett. 265: 85–87.

    Article  CAS  Google Scholar 

  20. Heather, J. S., S. J. Ferguson, D. J. Richardson, and S. Stephen (1993) The identification of a periplasmic nitrate reductase inParacoccus denitrificans.FEMS Microbiol Lett. 113: 107–112.

    Article  Google Scholar 

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Authors and Affiliations

  1. Interdisciplinary Program for Biochemical Engineering and Biotechnology, Semyung University, 390-711, Jecheon, Korea

    Seung Hoon Song & Young Je Yoo

  2. Enzy Biotech Inc., Technology Incubation Network, Semyung University, 390-711, Jecheon, Korea

    Sung Ho Yeom

  3. Department of Environmental Engineering, Semyung University, 390-711, Jecheon, Korea

    Suk Soon Choi

  4. School of Chemical Engineering, Seoul National University, 151-742, Seoul, Korea

    Young Je Yoo

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  1. Seung Hoon Song
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  2. Sung Ho Yeom
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  3. Suk Soon Choi
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  4. Young Je Yoo
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Correspondence to Young Je Yoo.

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Song, S.H., Yeom, S.H., Choi, S.S. et al. Effect of aeration on denitrification byOchrobactrum anthropi SY509. Biotechnol. Bioprocess Eng. 7, 352–356 (2002). https://doi.org/10.1007/BF02933520

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  • DOI: https://doi.org/10.1007/BF02933520

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