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International Journal of Pancreatology

, Volume 6, Issue 2, pp 109–118 | Cite as

Long-term effect of tumor necrosis factor and gamma interferon in human pancreatic carcinoma cells

  • Arthur B. Raitano
  • Philip Scuderi
  • Murray Korc
Article
  • 19 Downloads

Summary

The long-term cytotoxic/cytostatic effects of recombinant human tumor necrosis factor alpha (rhTNF) and gamma interferon (rhIFN-7) were studied in five human pancreatic carcinoma cell lines. The pancreatic tumor cell lines were heterogenous in their response to individual cytokines. During a 7-d incubation, MIA PaCa-2 cells were more sensitive to rhTNF than rhIFN-γ, whereas ASPC-1, T3M4, and COLO 357 cells were more sensitive to rhIFN-γ than rhTNF. PANC-1 cells were relatively insensitive to both cytokines. In a previous report, we demonstrated synergistic cytotoxic effects of rhTNF and rhIFN-γ during 48-h incubations in ASPC-1 and COLO 357 cells (Raitano et al,Pancreas, April, 1990). In this study, a 7-d treatment with both rhTNF and rhIFN-γ did not produce synergistic effects in any of the cell lines. However, a 24-h treatment with rhIFN-γ, followed by removal of the cytokine, markedly increased the long-term cytotoxic/cytostatic effects of rhTNF in ASPC-1, COLO 357, and T3M4 cells. In contrast, a similar pretreatment with rhTNF did not increase the long-term cytotoxic/cytostatic effects of rhIFN-γ in any of the cell lines. These data suggest that, in some human pancreatic carcinoma cell lines, rhIFN-γ may be especially useful in the long-term suppression of growth. Furthermore, brief pulses of rhIFN-γ may also be especially efficacious when followed by a subsequent prolonged exposure of cells to rhTNF.

Key Words

Cytokines cytotoxicity HLA-DR tumor cells 

Abbreviations

BSA

bovine serum albumin

FBS

fetal bovine serum

HBSS

Hank’s balanced salt solution

IFN-γ

gamma interferon

PBS

phosphate-buffered saline

rh

recombinant human

TFN

tumor necrosis factor

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Copyright information

© Humana Press Inc. 1990

Authors and Affiliations

  • Arthur B. Raitano
    • 1
    • 2
  • Philip Scuderi
    • 1
    • 2
  • Murray Korc
    • 1
    • 2
  1. 1.Departments of Microbiology and Immunology, Internal Medicine, Biochemistry, and the Arizona Cancer CenterUniversity of Arizona School of MedicineTuscon
  2. 2.Section of Endocrinology, Room 6411Arizona Health Sciences CenterTuscon

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