The growth conditions ofPantoea agglomerans, a phosphate solubilizing organism, were studied in our laboratory to determine the optimal conditions.Pantoea agglomerans showed the highest growth rate at 30°C, pH 7.0 and 2 vvm, after 50 h cultivation. A certain relationship between pH and phosphate concentration, was evident when the glucose concentration in the medium was changed. Increasing glucose concentration increased the pH buffer action of the broth. At glucose concentrations higher than the optimum concentration of 0.2 M, the cell growth was retarded.P. agglomerans consumed glucose as a substrate to produce organic acids which caused the pH decrease in the culture medium. The phosphate concentration in the medium was increased by the presence of the organic acids, which solubilized insoluble phosphates such as hydroxyapatite.
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Scheffer, F. and P. Schachtschabel (1992)Lehrbuch der Bodenkunde, Ferdinand Enke Verlag, Stuttgart, Germany.
Beever, R. E. and D. J. W. Burns (1980) Phosphorus uptake, sotorage and utilization by fungi.Adv. Bot. Res. 8: 127–219.
Moghimi, A. and M. E. Tate (1978) Does 2-ketogluconate chelated calcium in the pH in the pH range 2.4 to 6.4.Soil Biol. Biochem. 10: 289–292.
Suh, J.-S., Y.-S. Song, and K.-S. Kim (1995) Distribution of phosphate fractions in greenhouse soils located on southwest region in Korea.J. Kor. Soc. Soil Sci. 28: 270–277.
Park, B.-G., T.-H. Jeon, Y.-H. Kim, and Q.-S. Ho (1994) Status of farmers application rates of chemical fertilizer and farm manure for major crops.J. Kor. Soc. Soil Sci. 27: 238–246.
Park, B.-G., J.-H. Yoon, and Q.-S. Ho (1998) Comparison of several methods for the determination of available phosphorus in the soils for corn and rice.J. Kor. Soc. Soil Sci. 31: 1–8.
Suh, J.-S., S.-K. Lee, K.-S. Kim, and K.-Y. Seong (1995) Solubilization of insoluble phosphate byPseudomonas putida, Penicillium sp. andAspergillus niger isolated from Korean soils.J. Kor. Soc. Soil Sci. 28: 278–286.
Raj, J., D. J. Bagyaraj, and A. Manjunath (1981) Influence of soil inoculation with vesicular-arbuscular mycorrhiza and a phosphate dissolving bacterium on plant growth and32PTAKE.Soil Biol. Biochem. 13: 105–108.
Leheurte, F. and J. Berthelin (1988) Effect of a phosphate solubilizing bacteria on maize growth and root exudation over four levels of labile phosphorus.Plant Soil 105: 11–17.
Kim, K.-Y. (1997) Hydroxyapatite solubilization and organic acid production byEnterobacter agglomerans.J. Kor. Soc. Soil Sci. 30: 189–195.
Varsha, N. and H. H. Patel (2000)Aspergillus aculeatus as a rock phosphate solubilizer.Soil Biol. Biochem. 32: 559–565.
Louw, H. A. and D. M. Webley (1959) The bacteriology of the root region of the oat plant grown under controlled pot culture conditions.J. Appl. Bacteriol. 22: 216–226.
Alexander, M. (1977)Introduction to Soil Microbiology. John and Sons, New York, USA.
Sperber, J. I. (1958) The incidence of apatite-solubilizing organisms in the rhizosphere and soil.J. Agric. Res. 9: 778–781.
Shan, N. and P. Jelen (1990) Survival of lactic acid bacteria and their lactases under acidic conditions.J. Food Sci. 55: 506–509.
El-Gibaly, M. H., F. M. El-Reweiny, M. Abdel-Nasser, and T. A. El-Dahtory (1977) Studies on phosphate-solubilizing bacteria in soil and dissolvers and their morphological grouping.Zlb. Bakt. II. Abt. Bd. 132: 240–244.
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Jung, I., Park, DH. & Park, K. A study of the growth condition and solubilization of phosphate from hydroxyapatite byPantoea agglomerans . Biotechnol Bioproc E 7, 201–205 (2002). https://doi.org/10.1007/BF02932970
- Pantoea agglomerans
- 2-ketogluconic acid
- phosphate solubilization bacteria
- organic acid