A study of the growth condition and solubilization of phosphate from hydroxyapatite byPantoea agglomerans

Abstract

The growth conditions ofPantoea agglomerans, a phosphate solubilizing organism, were studied in our laboratory to determine the optimal conditions.Pantoea agglomerans showed the highest growth rate at 30°C, pH 7.0 and 2 vvm, after 50 h cultivation. A certain relationship between pH and phosphate concentration, was evident when the glucose concentration in the medium was changed. Increasing glucose concentration increased the pH buffer action of the broth. At glucose concentrations higher than the optimum concentration of 0.2 M, the cell growth was retarded.P. agglomerans consumed glucose as a substrate to produce organic acids which caused the pH decrease in the culture medium. The phosphate concentration in the medium was increased by the presence of the organic acids, which solubilized insoluble phosphates such as hydroxyapatite.

This is a preview of subscription content, access via your institution.

References

  1. [1]

    Scheffer, F. and P. Schachtschabel (1992)Lehrbuch der Bodenkunde, Ferdinand Enke Verlag, Stuttgart, Germany.

    Google Scholar 

  2. [2]

    Beever, R. E. and D. J. W. Burns (1980) Phosphorus uptake, sotorage and utilization by fungi.Adv. Bot. Res. 8: 127–219.

    Article  CAS  Google Scholar 

  3. [3]

    Moghimi, A. and M. E. Tate (1978) Does 2-ketogluconate chelated calcium in the pH in the pH range 2.4 to 6.4.Soil Biol. Biochem. 10: 289–292.

    Article  CAS  Google Scholar 

  4. [4]

    Suh, J.-S., Y.-S. Song, and K.-S. Kim (1995) Distribution of phosphate fractions in greenhouse soils located on southwest region in Korea.J. Kor. Soc. Soil Sci. 28: 270–277.

    Google Scholar 

  5. [5]

    Park, B.-G., T.-H. Jeon, Y.-H. Kim, and Q.-S. Ho (1994) Status of farmers application rates of chemical fertilizer and farm manure for major crops.J. Kor. Soc. Soil Sci. 27: 238–246.

    Google Scholar 

  6. [6]

    Park, B.-G., J.-H. Yoon, and Q.-S. Ho (1998) Comparison of several methods for the determination of available phosphorus in the soils for corn and rice.J. Kor. Soc. Soil Sci. 31: 1–8.

    Google Scholar 

  7. [7]

    Suh, J.-S., S.-K. Lee, K.-S. Kim, and K.-Y. Seong (1995) Solubilization of insoluble phosphate byPseudomonas putida, Penicillium sp. andAspergillus niger isolated from Korean soils.J. Kor. Soc. Soil Sci. 28: 278–286.

    Google Scholar 

  8. [8]

    Raj, J., D. J. Bagyaraj, and A. Manjunath (1981) Influence of soil inoculation with vesicular-arbuscular mycorrhiza and a phosphate dissolving bacterium on plant growth and32PTAKE.Soil Biol. Biochem. 13: 105–108.

    Article  CAS  Google Scholar 

  9. [9]

    Leheurte, F. and J. Berthelin (1988) Effect of a phosphate solubilizing bacteria on maize growth and root exudation over four levels of labile phosphorus.Plant Soil 105: 11–17.

    Article  Google Scholar 

  10. [10]

    Kim, K.-Y. (1997) Hydroxyapatite solubilization and organic acid production byEnterobacter agglomerans.J. Kor. Soc. Soil Sci. 30: 189–195.

    Google Scholar 

  11. [11]

    Varsha, N. and H. H. Patel (2000)Aspergillus aculeatus as a rock phosphate solubilizer.Soil Biol. Biochem. 32: 559–565.

    Article  Google Scholar 

  12. [12]

    Louw, H. A. and D. M. Webley (1959) The bacteriology of the root region of the oat plant grown under controlled pot culture conditions.J. Appl. Bacteriol. 22: 216–226.

    CAS  Google Scholar 

  13. [13]

    Alexander, M. (1977)Introduction to Soil Microbiology. John and Sons, New York, USA.

    Google Scholar 

  14. [14]

    Sperber, J. I. (1958) The incidence of apatite-solubilizing organisms in the rhizosphere and soil.J. Agric. Res. 9: 778–781.

    Article  CAS  Google Scholar 

  15. [15]

    Shan, N. and P. Jelen (1990) Survival of lactic acid bacteria and their lactases under acidic conditions.J. Food Sci. 55: 506–509.

    Article  Google Scholar 

  16. [16]

    El-Gibaly, M. H., F. M. El-Reweiny, M. Abdel-Nasser, and T. A. El-Dahtory (1977) Studies on phosphate-solubilizing bacteria in soil and dissolvers and their morphological grouping.Zlb. Bakt. II. Abt. Bd. 132: 240–244.

    CAS  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Don-Hee Park.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Jung, I., Park, DH. & Park, K. A study of the growth condition and solubilization of phosphate from hydroxyapatite byPantoea agglomerans . Biotechnol Bioproc E 7, 201–205 (2002). https://doi.org/10.1007/BF02932970

Download citation

Keywords

  • Pantoea agglomerans
  • hydroxyapatite
  • 2-ketogluconic acid
  • phosphate solubilization bacteria
  • organic acid