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A study of the growth condition and solubilization of phosphate from hydroxyapatite byPantoea agglomerans

Biotechnology and Bioprocess Engineering volume 7, pages 201–205 (2002)Cite this article

Abstract

The growth conditions ofPantoea agglomerans, a phosphate solubilizing organism, were studied in our laboratory to determine the optimal conditions.Pantoea agglomerans showed the highest growth rate at 30°C, pH 7.0 and 2 vvm, after 50 h cultivation. A certain relationship between pH and phosphate concentration, was evident when the glucose concentration in the medium was changed. Increasing glucose concentration increased the pH buffer action of the broth. At glucose concentrations higher than the optimum concentration of 0.2 M, the cell growth was retarded.P. agglomerans consumed glucose as a substrate to produce organic acids which caused the pH decrease in the culture medium. The phosphate concentration in the medium was increased by the presence of the organic acids, which solubilized insoluble phosphates such as hydroxyapatite.

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Authors and Affiliations

  1. Institute of Bioindustrial Technology, Chonnam National University, 500-757, Kwangju, Korea

    Il Jung, Don-Hee Park & Kyungmoon Park

  2. Faculty of Chemical Engineering, Chonnam National University, 500-757, Kwangju, Korea

    Il Jung & Don-Hee Park

  3. Research Institute for Catalysis, Chonnam National University, 500-757, Kwangju, Korea

    Don-Hee Park

  4. Eugene Science, 121-754, Seoul, Korea

    Kyungmoon Park

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  1. Il Jung
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  2. Don-Hee Park
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Correspondence to Don-Hee Park.

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Jung, I., Park, DH. & Park, K. A study of the growth condition and solubilization of phosphate from hydroxyapatite byPantoea agglomerans . Biotechnol Bioproc E 7, 201–205 (2002). https://doi.org/10.1007/BF02932970

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  • DOI: https://doi.org/10.1007/BF02932970

Keywords

  • Pantoea agglomerans
  • hydroxyapatite
  • 2-ketogluconic acid
  • phosphate solubilization bacteria
  • organic acid