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Metabolic flux analysis of a poly-β-hydroxybutyrate producing cyanobacterium,Synechococcus sp. MA19, grown under photoautotrophic conditions

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Abstract

To understand the utilization property of light energy,Synechococcus sp. MA19, a poly-β-hydroxybutyrate (PHB) producer, was cultivated at the different incident light intensities of 15.3, 50.0 and 78.2 W/m2 using media with and without phosphate. From the results of metabolic flux analysis, it was found that the cell yield based on ATP synthesis was estimated as 3.5×10−3 kg-biomass/mol-ATP in these cultures. Under the examined conditions, there were no significant differences in the efficiency of light energy conversion to chemical energies estimated as ATP synthesis and reducing potential (NADH+NADPH) formation whether the PHB synthesis took place or not. The energy converted from light to ATP was kept relatively high around the energy absorbed by the cells of 2.5–3.0×106 J h−1 kg−1, whereas the energy of reducing potential was hardly changed in the examined range of the energy absorbed by the cells.

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References

  1. Campbell, J. III, S. E. Stevens Jr., and D. L. Balkwill (1982) Accumulation of poly-β-hydroxybutyrate inSpirulina plantensis.J. Bacteriol. 149: 361–363.

    CAS  Google Scholar 

  2. Stal, L. J. (1992) Poly(hydroxyalkanoate) in cyanobacteria: an overview.FEMS Microbiol. Rev. 103: 169–180.

    Article  CAS  Google Scholar 

  3. Nishioka, M., K. Nakai, M. Miyake, Y. Asada, and M. Taya (2001) Production of poly-β-hydroxybutyrate by thermophilic cyanobacterium,Synechococcus sp. MA19, under phosphate-limited conditions.Biotechnol. Lett. 23: 1095–1099.

    Article  CAS  Google Scholar 

  4. Miyake, M., M. Erata, and Y. Asada (1996) A thermophilic cyanobacterium,Synechococcus sp. MA19, capable of accumulating poly-β-hydroxybutyrate.J. Ferment. Bioeng. 82: 512–514.

    Article  CAS  Google Scholar 

  5. Aiba, S. and M. Matsuoka (1979) Identification of metabolic model: citrate production from glucose byCandia lipolytica.Biotechnol. Bioeng. 21: 1373–1386.

    Article  CAS  Google Scholar 

  6. Vallino, J. J. and G. Stephanopoulos (1993) Metabolic flux distribution inCorynebacterium glutamicum during growth and lysine overproduction.Biotechnol. Bioeng. 41: 633–646.

    Article  CAS  Google Scholar 

  7. Shi, H., M. Shiraishi, and K. Shimizu (1997) Metabolic flux analysis of poly-(β-hydroxybutyric acid) inAlcali-genes astrophus from various carbon sources.J. Ferment. Bioeng. 84: 579–587.

    Article  CAS  Google Scholar 

  8. Takiguchi, N., H. Shimizu, and S. Shioya (1997) An on-line physiological state recognition system for the lysine fermentation process based on a metabolic reaction model.Biotechnol. Bioeng. 55: 170–181.

    Article  CAS  Google Scholar 

  9. Hua, Q., P. C. Fu, C. Yang, and K. Shimizu (1998) Microaerobic lysine fermentation and metabolic flux analysis.Biochem. Eng. J. 2: 89–100.

    Article  CAS  Google Scholar 

  10. Yang, C., Q. Hua, and K. Shimizu (2000) Energetics and carbon metabolism during growth of microalgal cells under photoautotrophic, mixotrophic and cyclic lightautotrophic/dark-heterotrophic conditions.Biochem. Eng. J. 6: 87–102.

    Article  CAS  Google Scholar 

  11. Yang, C., Q. Hua, and K. Shimizu (2002) Quantitative analysis of intracellular metabolic fluxes using GC-MS and two-dimensional NMR spectroscopy.J. Biosci. Bioeng. 93: 78–87.

    CAS  Google Scholar 

  12. Rippka, R., J. Deruelles, J. B. Waterbury, M. Herdman, and R. Y. Stanier (1979) Genetic assignments, strain histories and properties of pure cultures of cyanobacteria.J. Gen. Mcrobiol. 111: 1–61.

    Google Scholar 

  13. Hirata, S., J. Hata, M. Taya, and S. Tone (1997) Growth estimation ofSpirulina plantensis by considering light distribution in photoautotrophic batch culture.J. Chem. Eng. Jpn. 30: 355–359.

    Article  CAS  Google Scholar 

  14. Hata, J., Q. Hua, C. Yang, K. Shimizu, and M. Taya (2000) Characterization of energy conversion based on metabolic flux analysis in mixotrophic liverwort cells,Marchantia polymorpha.Biochem. Eng. J. 6: 65–74.

    Article  CAS  Google Scholar 

  15. Vallino, J. J. and G. Stephanopoulos (1990) Flux determination in cellular bioreaction networks: application to lysine fermentation. pp. 205–219 In: S. K. Sikdar, M. Bier, and P. Todd (eds.).Frontiers in Bioprocessing. CRC Press, Boca Raton, FL, USA.

    Google Scholar 

  16. Stumpf, P. K. and E. E. Conn (1980)The Biochemistry of Plants. Vol. 2. pp. 1–27. Academic Press, New York, NY, USA.

    Google Scholar 

  17. Holms, W. H. (1986) The central metabolic pathways ofEscherichia coli: relationship between flux and control at a branch point, efficiencies of conversion to biomass, and excretion of acetate.Curr. Topics Cell. Reg. 28: 69–105.

    CAS  Google Scholar 

  18. Ingraham, J. L., O. Maaloe, and F. C. Nedhardt (1983)Growth of the bacterial Cell. pp. 87–173. Sinauer Associates, Sunderland, MA, USA.

    Google Scholar 

  19. Rawn, J. D. (1989)Biochemistry. Neil Patterson, Publishers, Burlington, NC, USA.

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University, 560-8531, Toyonaka, Osaka, Japan

    Motomu Nishioka, Hajime Nishiuma & Masahito Taya

  2. Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology, 661-0974, Amagasaki, Hyogo, Japan

    Masato Miyake

  3. College of Science and Technology, Nihon University, 274-8501, Funabashi, Chiba, Japan

    Yasuo Asada

  4. Department of Biochemical Engineering and Science, Kyushu Institute of Technology, 820-0067, Iizuka, Fukuoka, Japan

    Kazuyuki Shimizu

Authors
  1. Motomu Nishioka
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  2. Hajime Nishiuma
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  3. Masato Miyake
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  4. Yasuo Asada
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  5. Kazuyuki Shimizu
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  6. Masahito Taya
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Correspondence to Masahito Taya.

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Nishioka, M., Nishiuma, H., Miyake, M. et al. Metabolic flux analysis of a poly-β-hydroxybutyrate producing cyanobacterium,Synechococcus sp. MA19, grown under photoautotrophic conditions. Biotechnol. Bioprocess Eng. 7, 295–302 (2002). https://doi.org/10.1007/BF02932839

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  • DOI: https://doi.org/10.1007/BF02932839

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