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Efficient transformation ofKlebsiella oxytoca by electroporation

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Abstract

A protocol for the transformation ofKlebsiella oxytoca by electroporation was developed. Preparation of competent cells at early exponential phase was most critical to obtain a high transformation efficiency. The highest efficiency of 1.6 × 106 transformants per μg DNA (pBR 322) could be obtained by electroporation ofK. oxytoca cells prepared at the OD600 of 0.2 with 1.25 μg DNA at the filed strength of 2.5 kV, the parallel resistance of 200 Ω and capacitance of 25 μF.

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Authors and Affiliations

  1. Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Kusong-dong, Yusong gu, 305-701, Taejon, Korea

    Ki Jun Jeong, Hyun Sook Lee, Sang Yup Lee & Yong Keun Chang

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  1. Ki Jun Jeong
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  2. Hyun Sook Lee
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  3. Sang Yup Lee
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  4. Yong Keun Chang
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Correspondence to Sang Yup Lee.

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Jeong, K.J., Lee, H.S., Lee, S.Y. et al. Efficient transformation ofKlebsiella oxytoca by electroporation. Biotechnol. Bioprocess Eng. 3, 48–49 (1998). https://doi.org/10.1007/BF02932484

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