Abstract
Binding characteristics ofBombyx mori nuclear polyhedrosis virus (BmNPV) toB. mori BmN-4 cells was determined. The cells had a single class of BmNPV-binding sites and displayed a low binding capacity for BmNPV in the range of 70 sites per cell. The biochemical nature of BmNPV-binding sites on the cell surface was identified. Treatment of BmN-4 cells with protease, wheat germ agglutinin, metabolic inhibitors, or lysosomotropic agents reduced BmNPV binding to BmN-4 cells, whereas treatment with phospholipase C showed no effect on virus binding. These results indicate that the structure of the binding site moiety is a protein-oligosaccharide complex.
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Kim, T.Y., Kang, H.C., Park, J.O. et al. Biochemical analysis of BmNPV attachment toBombyx mori BmN-4 cells. Biotechnol. Bioprocess Eng. 2, 59–61 (1997). https://doi.org/10.1007/BF02932465
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DOI: https://doi.org/10.1007/BF02932465