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Identification of genetic markers for Korean native cattle (Hanwoo) by RAPD analysis

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Abstract

In order to develop the specific genetic marker for Korean native cattle (Hanwoo), randomly amplified polymorphic DNA (RAPD) analysis of 6 different cattle breeds was attempted by using 38 decamer primers. In comparison of RAPD patterns, two distinctive DNA bands specific for Hanwoo were detected. One was 296 bp of DNA fragment found to be specific only for female Hanwoo when primer GTCCACACGG was employed. In individual analysis of this RAPD marker was observed only in female individuals with the possibility of 85.3%. The other was 521 bp of RAPD marker amplified using TCGGCGATAG and AGCCAGCGAA primers, which showed 83.0% of genetic frequency in 85 male and 68 female individuals tested. Nucleotide sequencing of these genetic markers revealed that 296 bp marker has a short microsatellite-like sequence, ACCACCACAC, and a tandem repeat sequence of microsatellite GAAAAATG in the determined sequence. Two distinctive tandem repeats of microsatellite sequences, AAC and GAAGA, were also appeared in 521 bp DNA marker. In BLAST search, any gene having high homology with these markers was not found

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Authors and Affiliations

  1. Department of Animal Sciences, College of Natural Resources, Yeungnam University, 712-749, Kyongsan, Korea

    Jung Sou Yeo

  2. Division of Pharmacy, College of Pharmacy, Yeungnam University, 712-749, Kyongsan, Korea

    Ji Sun Lee, Chang Hee Lee & Doo Hyun Nam

  3. Korea Food and Drug Administration, Eunpyung-ku, 122-704, Seoul, Korea

    Young Ja Jung

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  1. Jung Sou Yeo
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  2. Ji Sun Lee
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  3. Chang Hee Lee
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  5. Doo Hyun Nam
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Correspondence to Doo Hyun Nam.

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Yeo, J.S., Lee, J.S., Lee, C.H. et al. Identification of genetic markers for Korean native cattle (Hanwoo) by RAPD analysis. Biotechnol. Bioprocess Eng. 5, 23–26 (2000). https://doi.org/10.1007/BF02932348

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  • DOI: https://doi.org/10.1007/BF02932348

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