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Electrical recognition of label-free oligonucleotides upon streptavidin-modified electrode surfaces

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Abstract

For the purpose of developing a direct label-free electrochemical detection system, we have systematically investigated the electrochemical signatures of each step in the preparation procedure, from a bare gold electrode to the hybridization of label-free complementary DNA, for the streptavidin-modified electrode. For the purpose of this investigation, we obtained the following pertinent data; cyclic voltammogram measurements, electrochemical impedance spectra and square wave voltammogram measurements, in Fe(CN)6 3−/Fe(CN)6 4− solution (which was utilized as the electron transfer redox mediator). The oligonucleotide molecules on the streptavidin-modified electrodes exhibited intrinsic redox activity in the ferrocyanide-mediated electrochemical measurements. Furthermore, the investigation of electrochemical electron transfer, according to the sequence of oligonucleotide molecules, was also undertaken. This work demonstrates that direct label-free oligonucleotide electrical recognition, based on biofunctional streptavidin-modified gold electrodes, could lead to the development of a new biosensor protocol for the expansion of rapid, cost-effective detection systems.

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Authors and Affiliations

  1. The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, 567-0047, Ibaraki, Osaka, Japan

    Jong Wan Park, Ho Sub Jung, Hea Yeon Lee & Tomoji Kawai

  2. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST), 4-1-8 Honcho, 332-0012, Kawaguchi, Saitama, Japan

    Ho Sub Jung, Hea Yeon Lee & Tomoji Kawai

Authors
  1. Jong Wan Park
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  2. Ho Sub Jung
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  3. Hea Yeon Lee
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  4. Tomoji Kawai
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Correspondence to Hea Yeon Lee.

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Park, J.W., Jung, H.S., Lee, H.Y. et al. Electrical recognition of label-free oligonucleotides upon streptavidin-modified electrode surfaces. Biotechnol. Bioprocess Eng. 10, 505–509 (2005). https://doi.org/10.1007/BF02932285

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  • DOI: https://doi.org/10.1007/BF02932285

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