Abstract
The chemical, physical, and emulsifying properties of BSF-1, which is an extracellular lipopolysaccharide biosurfactant produced byKlebsiella oxytoca strain BSF-1, were studied. BSF-1 was found to be composed mainly of carbohydrate and fatty acids. The average molecular weight was 1,700–2,000 kDa. The polysaccharide fraction containedl-rhamnose,d-galactose,d-glucose, andd-glucuronic acid at a molar ratio of 3∶1∶1∶1. The fatty acid content was 1.1% (w/w) and consisted mainly of palmitic acid (C16∶0), 3-hydroxylauric acid (3-OH-C12∶0), and lauric acid (C12∶0). In terms of thermal properties, BSF-1 was revealed to have inter- and intra-molecular hydrogen bonds. The hydrodynamic volume (intrinsic viscosity) of BSF-1 was 22.8 dL/g. BSF-1 could be maintained as a stable emulsion for 48 h through a low-level reduction in surface tension. The optimal emulsification temperature was 30°C. Emulsification by BSF-1 was efficient at both acidic and neutral pH values.
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Kim, P., Kim, J.H. Characterization of a novel lipopolysaccharide biosurfactant fromKlebsiella oxitoca . Biotechnol. Bioprocess Eng. 10, 494–499 (2005). https://doi.org/10.1007/BF02932283
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DOI: https://doi.org/10.1007/BF02932283