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Expression patterns of anArabidopsis phenylalanine ammonia-lyase promoter in transgenic tobacco

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Abstract

We have cloned and characterized one of the genes that encode phenylalanine ammonia-lyase fromArabidopsis thaliana. A 1.8-kb fragment containing the upstream region of this gene,PALI, was transcriptionally fused to the ß-glucuronidase reporter gene, and the construct was introduced into tobacco byAgrobacterium-mediated leaf disc transformation. The distribution of ß-glucuronidase activity in the transgenic plants was analysed histochemically. The spatial pattern of activity showed that thePALI promoter is very active in the vascular tissues. No expression was observed in epidermal cells, trichomes and apical meristems. Activation of thePALI promoter in the early stages of seedling development remained confined to the regions adjacent to the root apical meristems; in later stages of seedling development, the pattern of expression was drastically altered. The results suggest that thePALI promoter is under the control of a complex set of signal transduction pathways which lead to its activation in response to tissue-specific, wounding and developmental cues.

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Authors and Affiliations

  1. Molecular Genetics Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110 067, New Delhi, India

    S. V. Naga Prasad, M. Thungapathra, Vindhya Mohindra & Kailash C. Upadhyaya

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  1. S. V. Naga Prasad
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  2. M. Thungapathra
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  3. Vindhya Mohindra
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  4. Kailash C. Upadhyaya
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Prasad, S.V.N., Thungapathra, M., Mohindra, V. et al. Expression patterns of anArabidopsis phenylalanine ammonia-lyase promoter in transgenic tobacco. J. Genet. 74, 111–126 (1995). https://doi.org/10.1007/BF02932197

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  • DOI: https://doi.org/10.1007/BF02932197

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