Summary
Nucleolar persistence in metaphase plates is a feature observed in most of the cells in neoplastic processes. Pathological persistence or fragmentation of the nucleoli is thought to be the cause of some numerical chromosomal aberrations due to non-disjunction of the chromatids, with particular involvement of the satellite chromosomes. Thus, a combined selective staining of both the nucleoli (amido black 10B according to Mundkur and Brauer's cytochemical technique) and the chromosomes (neutral red) was applied to the metaphase plates of patients with chronic myeloid leukaemia in the blastic crisis. Duplicated Ph1 was associated with amido black-stained areas at a very high rate in some cases. Since the blastic crisis in chronic myeloid leukaemia is
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This work was supported in part by a grant from theConsiglio Nazionale delle Ricerche (CNR), Roma, Italy, «Progetto finalizzato controllo della crescita neoplastica», research contract no. 79.00613.96.
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Castoldi, G., Borsetti, G., Abbasciano, V. et al. Persistence of nucleolar RNA-rich structures and Ph1 duplication in the blastic crisis of chronic myeloid leukaemia. La Ricerca Clin. Lab. 9, 265–271 (1979). https://doi.org/10.1007/BF02932178
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DOI: https://doi.org/10.1007/BF02932178