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Primer R108 performs best in the RAPD strain typing of threeAspergillus species frequently isolated from patients

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Abstract

We evaluated the suitability of various primers for the RAPD (randomamplifiedpolymorphicDNA) accurate species identification and strain typing ofAspergillus clinical isolates. Five primers described previously were tested for their discriminatory power in threeAspergillus species (A. fumigatus, A. niger agg. andA. flavus — 23 clinical isolates and 2 reference strains). Clustering of RAPD fingerprints corresponded well with the identification based on morphological features. All isolates were resolved as different strains using the primer R108 and the RAPD protocol optimized for a Robocycler thermal cycler. RAPD with the primer R108 thus can be considered to be a valuable, simple and powerful tool for identification and strain delineation ofAspergillus spp.

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Authors and Affiliations

  1. Institute of Microbiology, Palacký University, 775 15, Olomouc, Czechia

    V. Raclavský & P. Hamal

  2. Institute of Biology, Faculty of Medicine, Palacký University, 775 15, Olomouc, Czechia

    J. Trtková & L. Rusková

  3. Department of Clinical Microbiology, University Hospital, Hradec Králové, Czechia

    V. Buchta

  4. Department of Clinical Biochemistry, University Hospital, Hradec Králové, Czechia

    R. Bolehovská

  5. Institute of Epidemiology, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czechia

    M. Vacková

Authors
  1. V. Raclavský
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  2. J. Trtková
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  3. L. Rusková
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  4. V. Buchta
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  5. R. Bolehovská
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  6. M. Vacková
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  7. P. Hamal
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Corresponding author

Correspondence to V. Raclavský.

Additional information

This work was supported by theMinistry of Health of the Czech Republic (grant no. NI/7498-3).

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Raclavský, V., Trtková, J., Rusková, L. et al. Primer R108 performs best in the RAPD strain typing of threeAspergillus species frequently isolated from patients. Folia Microbiol 51, 136–140 (2006). https://doi.org/10.1007/BF02932169

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  • DOI: https://doi.org/10.1007/BF02932169

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