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Effect of oral application of a probioticE. coli strain on the intestinal microflora of children of allergic mothers during the first year of life

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Influence of intestinal colonization by a probioticE. coli strain on the incidence of bacterial pathogens in stool and allergic symptoms during the 1st year of life was monitored in 3 groups: colonized children of allergic mothers (AC;n=52), noncolonized children of allergic mothers (AN;n=50), children of nonallergic mothers (NC;n=42). Colinfant vaccine was administered within 2 d after birth, 3× a week over a period of 4 weeks. Stool samples were examined after 2 d and at the age of 3, 6 and 12 months. At 3 monthsE. coli was present in 90 %, at 12 months in 73 % of AC. Pathogens were significantly less frequent on day 3 and at 3 months in ACvs. AN (15vs. 61 %,p<0.001; 15vs. 38 %,p<0.01) andvs. NC (15vs. 63 %,p<0.001; 15vs. 53 %,p<0.001). AC exhibited lower incidence ofStaphylococcus epidermidis than AN on day 3 (6vs. 31 %,p<0.001) and ofKlebsiella strains on day 3 and at 3 months (4vs. 20 %,p<0.05; 5vs. 24 %,p<0.01). AC showed a lower incidence ofPseudomonas aeruginosa than NC on day 3 (6vs. 31 %,p<0.01) andKlebsiella spp. on day 3 and at 3 months (4vs. 22 %,p<0.05; 5vs. 45 %,p<0.001). No significant differences were recorded after 6 and 12 months. The incidence of allergies was 3 % in AC, 26 % in AN (p<0.01), and 10 % in NC.

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colonized children of allergic mothers


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noncolonized children of allergic mothers


transforming growth factor β


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Correspondence to I. Kocourková.

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This work was supported by theMinistry of Public Health of the Czech Republic (Internal Grant Agency project 7525-3).

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Kocourková, I., Žádníková, R., Žižka, J. et al. Effect of oral application of a probioticE. coli strain on the intestinal microflora of children of allergic mothers during the first year of life. Folia Microbiol 52, 189–193 (2007).

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