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Zygnematalean zygospores: Morphological features and use in species identification

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Abstract

Suitable morphological characteristics for identification of zygnematalean algae were determined using a combination of classical light microscopy (LM) techniques, fluorescence microscopy (with blue and green excitation), scanning electron microscopy (SEM) and specialized culture methods. Characteristics of spore germination, growth and reproduction under culture conditions identifiedZygnema chalybeospermum in a mixture of zygnematalean spores collected from a small fishpond in Czechia. Reproduction in general, particularly aplanospore formation and lateral conjugation was more frequent and occurred earlier in a nutrient poor medium than in a nutrient rich medium, where vegetative growth was more vigorous. Variability in spore size was wide and influenced by the origin (sexual and/or asexual) of the spores. Asexual spores, particularly partenospores were rounded and significantly smaller than sexual ones. Thus spore morphology alone (size and shape) is not a particularly helpful characteristic for species identification. The mesospore of mature spores contained lipids and a sporopolenin-like material (algaenan), which showed green autofluorescence with blue excitation. The mesospore ornamentation, the only characteristic found that is suitable for identification purposes, can be observed easily in LM and SEM after exospore removal by KOH treatment. Detailed LM and SEM observations of the zygospores of allZygnema species thus could provide basic data necessary for the preparation of an atlas and key for species identification which, after completion with molecular methods, brings clarification into the genetic relationships between morphospecies.

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Abbreviations

DIC:

differential interference contrast (Nomarski contrast)

FM:

fluorescence microscopy

LM:

light microscopy

SEM:

scanning electron microscopy

References

  • Allen M.A.: The biology of a species complex inSpirogyra. PhD Thesis. Indiana University, Bloomington (USA) 1958.

    Google Scholar 

  • Czurda V.: Experimentelle Untersuchungen über die Sexualitätsverhältnisse der Zygnemalen.Bot.Zbl.47, 15–68 (1930).

    Google Scholar 

  • Czurda V.: Experimentelle Analyse der kopulationsauslösenden Bedingungen bei Mikroorganismen. I. Untersuchungen an Algen (Spirogyra, Zygnema undHyalotheca).Bot.Zbl.51, 711–762 (1933).

    Google Scholar 

  • Ferrer N.C., Caceres E.J.:Spirogyra salmonispora sp.nov. (Zygnematophyceae, Chlorophyta): a new freshwater species of the sectionConjugata.Arch.Protistenkd.146, 101–106 (1995).

    Google Scholar 

  • Graham L.E., Wilcox L.W.:Algae. Prentice Hall, Upper Saddle River (USA) 2000.

  • Guillard R.L., Lorenzen C.L.: Yellow-green algae with chlorophyllidec.J.Phycol.8, 10–14 (1972).

    CAS  Google Scholar 

  • Gupta S., Agrawal S.C.: Survival of blue-green and green algae under stress conditions.Folia Microbiol.51, 121–128 (2006).

    Article  CAS  Google Scholar 

  • Hansgirg A.:Preliminary Introduction to Freshwater Algae II. (In Czech) Natural History Research Archive of Bohemia, Prague 1892.

    Google Scholar 

  • Hoshaw R.W., Wang J.C., McCourt R.M., Hull H.M.: Ploidal changes in clonal cultures ofSpirogyra communis and implications for species definition.Amer.J.Bot.72, 1005–1011 (1985).

    Article  Google Scholar 

  • Kadlubowska J.Z.:Conjugatophyceae I.Chlorophyta VIII.Zygnemales, pp. 1–531 in H. Ettl, J. Gerloff, H. Heynig, D. Mollenhauer (Eds):Süsswasserflora von Mitteleuropa 16. Gustav-Fischer-Verlag, Stuttgart (Germany) 1984.

    Google Scholar 

  • Kadlubowska J.Z.: Remarks on EuropeanZygnemales. Phytomorphology Golden Jubilee Issue 457–466 (2001).

  • Lewis S.H., Entwisle T.J.:Spirogyra cardinia (Zygnemataceae, Zygnematophyceae, Chlorophyta): a new species of freshwater alga from Victoria, Australia.Muelleria14, 99–102 (2000).

    Google Scholar 

  • Mann D.G.: The species concept in diatoms (Phycological Reviews 18).Phycologia38, 437–495 (1999).

    Article  Google Scholar 

  • McCourt R.M., Hoshaw R.W.: Noncorrespondence of breeding groups, morphology and monophyletic groups inSpirogyra (Zygnemataceae, Chlorophyta) and the application of species concepts.Syst.Bot.15, 69–78 (1990).

    Article  Google Scholar 

  • McCourt R.M., Karol K.G., Kaplan S., Hoshaw R.W.: UsingrycL sequences to test hypothese of chloroplast and thallus evolution in the conjugating green algae (Zygnematales, Charophyceae).J.Phycol.31, 989–995 (1995).

    Article  CAS  Google Scholar 

  • Novis P.M.: New records ofSpirogyra andZygnema (Charophyceae, Chlorophyta) in New Zealand.N.Zeeland J.Bot.42, 139–152 (2004).

    Google Scholar 

  • Poulíčková A., Lhotský O., Dřímalová D.: Review of cyanobacteria and algae of the Czech Republic.Czech Phycol.4, 19–35 (2004).

    Google Scholar 

  • Staub R.: Ernährungsphysiologisch-autökologische Untersuchungen an der planktonischen BlaualgeOscillatoria rubescens DC.Schweiz.Z.Hydrol.23, 82–198 (1961).

    Article  Google Scholar 

  • Van den Hoek C., Mann D.G., Jahns H.M.:Algae. An Introduction to Phycology. Cambridge University Press, Cambridge (UK) 1993.

    Google Scholar 

  • Versteegh G.J.M., Blokker P.: Resistant macromolecules of extant and fossil microalgae.Phycol.Res.52, 325–339 (2004).

    Article  CAS  Google Scholar 

  • Vítová M., Hendrychová J., Cepák V., Zachleder V.: Visualization of DNA-containing structures in various species ofChlorophyta, Rhodophyta andCyanophyta using SYBR Green I dye.Folia Microbiol.50, 333–340 (2005).

    Article  Google Scholar 

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Correspondence to A. Poulíčková.

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This work was supported by grants (522/03/0323 and 206/07/0115) of theGrant Agency of the Czech Republic, andInstitutional Research Concept of the Institute of Microbiology (AV 0Z 5020 0510).

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Poulíčková, A., Žižka, Z., Hašler, P. et al. Zygnematalean zygospores: Morphological features and use in species identification. Folia Microbiol 52, 135–145 (2007). https://doi.org/10.1007/BF02932152

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  • DOI: https://doi.org/10.1007/BF02932152

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