Folia Microbiologica

, Volume 52, Issue 1, pp 95–98 | Cite as

Effect of root canal sealers on mouse peritoneal macrophage functions



Three root canal filling materials,viz. calcium hydroxide-based cement (Apexit®, resin-based cement (AH-plus®) and glass-ionomer based material (Ketac Endo®) were tested for their influence on several functions of peritoneal macrophages from Balb/c mice. Macrophage functions were evaluated by the adherence, phagocytic, candidacidal and Nitro blue tetrazolium-dye assays. Ketac-Endo® enhanced all macrophage functions in the first 2 d (p≤0.05), when compared to the positive control, but this effect had changed after 7 and 14 d, causing inhibition of these functions. Other materials suppressed substrate adherence capacity and phagocytosis, while significantly stimulating macrophage microbicidal activity (p≤0.05) in a time-dependent manner.


Root Canal Calcium Hydroxide Root Resorption Mouse Peritoneal Macrophage Killing Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



adherence index



Nitroblue tetrazolium


phagocytic index




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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2007

Authors and Affiliations

  • I. Brekalo
    • 1
  • S. Pezelj-Ribarić
    • 2
  • M. Abram
    • 3
  • V. Ahel
    • 4
  1. 1.Department of Dental PathologyUniversity of RijekaRijekaCroatia
  2. 2.Department of Oral MedicineUniversity of RijekaRijekaCroatia
  3. 3.Department of Microbiology, Medical FacultyUniversity of RijekaRijekaCroatia
  4. 4.Department of Oral and Maxillofacial SurgeryUniversity Hospital RijekaCroatia

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