Folia Microbiologica

, Volume 52, Issue 1, pp 95–98 | Cite as

Effect of root canal sealers on mouse peritoneal macrophage functions

Article

Abstract

Three root canal filling materials,viz. calcium hydroxide-based cement (Apexit®, resin-based cement (AH-plus®) and glass-ionomer based material (Ketac Endo®) were tested for their influence on several functions of peritoneal macrophages from Balb/c mice. Macrophage functions were evaluated by the adherence, phagocytic, candidacidal and Nitro blue tetrazolium-dye assays. Ketac-Endo® enhanced all macrophage functions in the first 2 d (p≤0.05), when compared to the positive control, but this effect had changed after 7 and 14 d, causing inhibition of these functions. Other materials suppressed substrate adherence capacity and phagocytosis, while significantly stimulating macrophage microbicidal activity (p≤0.05) in a time-dependent manner.

Abbreviations

AI

adherence index

macrophage(s)

NBT

Nitroblue tetrazolium

PI

phagocytic index

TGC

thioglycollate

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2007

Authors and Affiliations

  • I. Brekalo
    • 1
  • S. Pezelj-Ribarić
    • 2
  • M. Abram
    • 3
  • V. Ahel
    • 4
  1. 1.Department of Dental PathologyUniversity of RijekaRijekaCroatia
  2. 2.Department of Oral MedicineUniversity of RijekaRijekaCroatia
  3. 3.Department of Microbiology, Medical FacultyUniversity of RijekaRijekaCroatia
  4. 4.Department of Oral and Maxillofacial SurgeryUniversity Hospital RijekaCroatia

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