Abstract
Helicobacter pylori from patients with different diseases, including so-called autoimmune thyroiditis, chronic tonsillitis and tonsillar cancer, was isolated and cultured. It was identified according to the genotype using labeled hybridization probes complementary to six sequences ofcagA andvacA genes. Different types of strains were found in isolates from gastrointestinal tract and patients suffering from thyroiditis. Six out of seven genotyped isolates from patients in ourDepartment of Otorhinolaryngology and Head and Neck Surgery exhibited the same genotype, differing from isolates obtained from other patients; the 7th isolate originated from a patient who had undergone surgery for deviatio septi nasi, at the same time suffering from autoimmune thyroiditis, having confirmed gastric infection byH. pylori from biopsy. This data made it possible to formulate the hypothesis on probable association of specificH. pylori genotype with chronic tonsillitis and tonsillar cancer. We assessed commercial transport media and improved nucleic acid isolation techniques and the RT-PCR-based tests, which allowed us to skip a culture step and to test directly the patients’ samples; however, for ful confirmation of our hypothesis and explanation of possible mechanisms of the contribution ofHelicobacter sp. to the pathogenesis of the disease further data are to be collected and evaluated.
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Abbreviations
- AT:
-
autoimmune thyroiditis
- GIT:
-
gastrointestinal tract
- MWP:
-
microwell plate
- NA:
-
nucleic acid(s)
- ORL:
-
otorhinolaryngology
- OSAS:
-
obstructive sleep apnoe syndrome
- PAI:
-
pathogenicity island(s)
- PCR:
-
polymerase chain reaction
- RT-PCR:
-
real time PCR
- RUT:
-
rapid urea test
- UBT:
-
urea breath test
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The study was supported by grants 8115-3 and 9077-3 of theInternal Grant Agency of the Ministry of Health of the Czech Republic and VZ-MSM of theMinistry of Education, Youth and Sports of the Czech Republic.
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Pavlík, E., Lukeš, P., Potužníková, B. et al. Helicobacter pylori isolated from patients with tonsillar cancer or tonsillitis chronica could be of different genotype compared to isolates from gastrointestinal tract. Folia Microbiol 52, 91–94 (2007). https://doi.org/10.1007/BF02932145
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DOI: https://doi.org/10.1007/BF02932145