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Neisseria meningitidis strains from patients with invasive meningococcal disease differ in stimulation of cytokine production

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Abstract

Cytokine production was determinedin vitro after stimulation with three differentNeisseria meningitidis (NM) strains. Virulent NM B strain isolated from a patient with mild course of invasive meningococcal disease (IMD) elicited higher cytokine production than NM B and NM C hypervirulent strains isolated from patients with moderate and fatal course of IMD, respectively. Endotoxin concentration afterin vitro stimulation correlated with cytokine production: the highest endotoxin levels were observed with virulent NM B strain. Serum cytokines and endotoxin levels showed an opposite trend. These results suggest that inflammatory response during IMD is predominantly influenced by host factors.

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Abbreviations

APACHE:

acute physiological and chronic health evaluation

CRP:

C-reactive protein

FC:

flow cytometer

FMS:

fulminant meningococcal sepsis

IL:

interleukin

IMD:

invasive meningococcal disease

LOS:

lipooligosaccharide

MLST:

multilocus sequence typing

NM:

Neisseria meningitidis

rSBA:

serum bactericidal assay with the use of baby rabbit complement

SOFA:

sepsis organ failure assessment

TNF-α:

tumor necrosis factor α

WB:

whole blood

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Authors and Affiliations

  1. 3rd Department of Infectious and Tropical Diseases, 1st Faculty of Medicine, Charles University in Prague, Prague, Czechia

    M. Holub, M. Scheinostová, A. Fišerová & O. Beran

  2. Department of Infectious Diseases, 3rd Faculty of Medicine, Charles University in Prague, Prague, Czechia

    O. Džupová

  3. National Reference Laboratory for Meningococcal Infections, National Institute of Public Health, Prague, Czechia

    J. Kalmusová, M. Musílek & P. Křížová

Authors
  1. M. Holub
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  2. M. Scheinostová
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  3. O. Džupová
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  4. A. Fišerová
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  5. O. Beran
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  6. J. Kalmusová
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  7. M. Musílek
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  8. P. Křížová
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Corresponding author

Correspondence to M. Holub.

Additional information

The study is supported by grants IGA MZ ČR NR/8014-3 and IGA MZ ČR NR/1A8688-3/2005 of theMinistry of Public Health of the Czech Republic and grant MŠM 002 162 0806 of theMinistry of Education, Youth and Sports of the Czech Republic.

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Holub, M., Scheinostová, M., Džupová, O. et al. Neisseria meningitidis strains from patients with invasive meningococcal disease differ in stimulation of cytokine production. Folia Microbiol 52, 525–528 (2007). https://doi.org/10.1007/BF02932114

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  • DOI: https://doi.org/10.1007/BF02932114

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