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Decreased susceptibility to antifungals in respiratory-deficientKluyveromyces lactis mutants

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Abstract

Decreased susceptibility ofK. lactis mutants impaired in the function of cytochromec, cytochromec 1 and cytochrome-c oxidase to fluconazole, bifonazole and amphotericin B in comparison with the isogenic wild-type strain was observed. Flow cytometry with rhodamine 6G did not show any changes in the accumulation of the dye in the mutant cells compared with the corresponding wild-type strain. Sterol analysis showed similar overall amount of sterols in both wild-type and mutant cells. Taking into account the increased amphotericin B resistance and significantly diminished susceptibility of mutant cells to lyticase digestion, the cell wall structure and/or composition may probably be responsible for the observed changes in the susceptibility of mutants to the antifungal compounds used.

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Abbreviations

AMB:

amphotericin B

BIF:

bifonazole

FACS:

fluorescence-activated cell sorter

YNB:

yeast nitrogen base (medium)

YPD:

yeast extract-peptone dextrose

ITR:

itraconazole

FLC:

fluconazole

NYS:

nystatin

R6G:

rhodamine 6G

GC:

gas chromatography

YNB MM:

YNB minimal medium

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Authors and Affiliations

  1. Department of Microbiology and Virology, Comenius University in Bratislava, 842 15, Bratislava, Slovakia

    M. Šarinová, V. Straková, K. Balková & Y. Gbelská

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  1. M. Šarinová
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  2. V. Straková
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  3. K. Balková
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  4. Y. Gbelská
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Correspondence to Y. Gbelská.

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This work was supported by grant no. 1/2338/05 from theSlovak Grant Agency of Science VEGA and grants of theComenius University in Bratislava UK/129/07 and UK/145/07.

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Šarinová, M., Straková, V., Balková, K. et al. Decreased susceptibility to antifungals in respiratory-deficientKluyveromyces lactis mutants. Folia Microbiol 52, 484–490 (2007). https://doi.org/10.1007/BF02932108

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  • DOI: https://doi.org/10.1007/BF02932108

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