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Alterations in mitochondrial morphology ofSchizosaccharomyces pombe induced by cell-death promoting agents

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Abstract

The effect of the yeast cell-death inducing agents, Bax and acetic acid, on mitochondrial structure ofSchizosaccharomyces pombe was studied. Comparison of mitochondrial structures in cells grown on different substrates and visualized with different probes revealed variations in their morphology. Cells grown on respiratory C sources as well as in the presence of antimycin A exhibited punctuated mitochondria when visualized with mitochondrially targeted green fluorescent protein, while they still appeared as tubular structures when stained with DiOC6(3). Both expression of Bax and acetic acid treatment induced fragmentation and aggregation of mitochondrial network, which could be prevented by coexpression of Bcl-XL. Aberrant mitochondrial morphology generated by either Bax or acetic acid was not accompanied with the loss of mitochondrial genome (mtDNA), indicating that alterations of mitochondrial morphology following death stimuli follow different mechanisms than those involved in mitochondrial inheritance mutants.

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Abbreviations

DAPI:

4′,6-diamidino-2-phenylindole

DiOC6(3):

3-hexyl-2-[3″-(3′-hexyl-2′(3′H)benzoxazolylidene)-l″-propenyl]benzoxazolium iodide (see formula)

FCS:

fermentable carbon source(s)

MT:

mitochondrial(ly)

mtGFP:

MT-targeted green fluorescent protein

NCS:

nonfermentable carbon source(s)

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Comenius University, 842 15, Bratislava, Slovakia

    V. Pevala, J. Kolarov & P. Polčic

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  1. V. Pevala
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  2. J. Kolarov
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  3. P. Polčic
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Correspondence to P. Polčic.

Additional information

This work was supported by grants from theAgency for Support of Research and Development no. APVT-20-012404 and no. APVT-20-017202 and fromNATO no. RIG 981468.

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Pevala, V., Kolarov, J. & Polčic, P. Alterations in mitochondrial morphology ofSchizosaccharomyces pombe induced by cell-death promoting agents. Folia Microbiol 52, 381–390 (2007). https://doi.org/10.1007/BF02932093

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  • DOI: https://doi.org/10.1007/BF02932093

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