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Optimization of γ-polyglutamic acid production byBacillus subtilis ZJU-7 using a surface-response methodology

Abstract

The components of the media used to elicit the biosynthesis of poly-γ-glutamic acid (γ-PGA) byBacillus subtilis ZJU-7 were investigated, particularly the carbon and nitrogen sources. Of the 7 carbon sources investigated, sucrose induced the highest rate of γ-PGA productivity; among the nitrogen sources, tryptone had the best effect for γ-PGA production. A 26−2 fractional factorial design was used to screen factors that influence γ-PGA production significantly, and a central composite design was finally adopted to formulate the optimal medium. γ-PGA productivity improved approximately 2-fold when the optimal medium was used compared with the original nonoptimized medium, and volumetric productivity reached a maximum of 58.2 g/L after a 24-h cultivation period.

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Correspondence to Zhinan Xu.

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Shi, F., Xu, Z. & Cen, P. Optimization of γ-polyglutamic acid production byBacillus subtilis ZJU-7 using a surface-response methodology. Biotechnol. Bioprocess Eng. 11, 251–257 (2006). https://doi.org/10.1007/BF02932039

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  • DOI: https://doi.org/10.1007/BF02932039

Keywords

  • poly-γ-glutamic acid
  • l-glutamic acid
  • Bacillus subtilis
  • response surface methodology