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Studies on decolorization process for rhEGF as cosmetic ingredient

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Abstract

A decolorization process using ion exchange chromatography was developed to refine rhEGF as a cosmetic ingredient. A macroreticular resin (D314) was selected, with respect to its high decolorization rate and recovery yield of rhEGF, and the operational conditions of the decolorization process optimized. The optimum conditions were as follows: the rhEGF effluent was ion exchanged at a flow rate of 60.0 mL/h, with an effluent pH 5.0, using a chromatographic column (i.d. 16mm) packed with D314, with a 7.5 cm in bed height. The decolorization process was carried out under the optimum conditions, and halted when the effluent volume reached 350 mL, giving a decolorization rate and recovery yield of rhEGF higher than 67 and 80%, respectively. When the decolorization rate exceeded 67%, the final product turned out to be white or light yellowish, which was to the satisfaction of the cosmetic standard.

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Authors and Affiliations

  1. School of Applied Technologies, Wenzhou University, 325035, Wenzhou, China

    Xiao-wei Zhao & Mao-hong Zhou

  2. Institute of bioengineering, Department of Chemical Engineering and Bioengineering, Zhejiang University, 310027, Hangzhou, China

    Zhi-nan Xu & Pei-lin Cen

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  1. Xiao-wei Zhao
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  2. Zhi-nan Xu
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  3. Mao-hong Zhou
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  4. Pei-lin Cen
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Correspondence to Zhi-nan Xu.

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Zhao, Xw., Xu, Zn., Zhou, Mh. et al. Studies on decolorization process for rhEGF as cosmetic ingredient. Biotechnol. Bioprocess Eng. 10, 236–241 (2005). https://doi.org/10.1007/BF02932019

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  • DOI: https://doi.org/10.1007/BF02932019

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