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Direct triazine herbicide detection using a self-assembled photosynthetic reaction center from purple bacterium

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Abstract

In this study, a direct detection system for triazine derivative herbicides was developed using the photosynthetic reaction center (RC) from the purple bacterium,Rhodobacter sphaeroides, and surface plasmon resonance (SPR) apparatus. The histidine-tagged RCs were immobilized on an SPR gold chip using nickel-nitrilotriacetic acid groups as a binder for one of the triazine herbicide, atrazine. The SPR responses were proportional to the sample concentrations of atrazine in the range 0.1–1 μg/mL. The sensitivity of the direct detection of atrazine using the RC-assembled sensor chip was higher than that using the antibody-immobilized chip. The other types of herbicides, DCMU or MCPP, were not detected with such high sensitivity. The results indicated the high binding selectivity of the RC complex.

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Abbreviations

DCMU:

N'-(3,4-Dichlorophenyl)-N,N-dimethylurea

ELISA:

Enzyme-linked immunosorbent assay

HHisRC:

Heavy-subunit-histidine-tagged RC

IgG:

Immunoglobulin G

MCPP:

2-(2-Methyl-4-chlorophenoxy) propionic acid

Ni-NTA:

Nickel-nitrilotriacetic acid

PSII:

Photosystem II

QB :

Secondary quinone of PSII or RC

RC:

Photosynthetic reaction center

SPR:

Surface plasmon resonance

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Authors and Affiliations

  1. National Institute for Advanced Interdisciplinary Research, AIST, MITI 1-1-4 Higashi, 305-8562, Tsukuba, Ibaraki, Japan

    Chikashi Nakamura, Miki Hasegawa, Kazumi Shimada & Jun Miyake

  2. Division of Biotechnology School of Agriculture, Ibaraki University, 300-0331, Ami, Ibaraki, Japan

    Kazumi Shimada & Makoto Shirai

Authors
  1. Chikashi Nakamura
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  2. Miki Hasegawa
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  3. Kazumi Shimada
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  4. Makoto Shirai
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  5. Jun Miyake
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Correspondence to Chikashi Nakamura.

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Nakamura, C., Hasegawa, M., Shimada, K. et al. Direct triazine herbicide detection using a self-assembled photosynthetic reaction center from purple bacterium. Biotechnol. Bioprocess Eng. 5, 413–417 (2000). https://doi.org/10.1007/BF02931940

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  • DOI: https://doi.org/10.1007/BF02931940

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