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Clearance of false-positive antigen-antibody reactions of a diagnostic antigen produced inEscherichia coli with human sera

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Abstract

Although many pharmaceutically useful proteins are produced inE. coli expression system, it is very rare for the system to be used in the production of diagnostic antigen due to a major problem,i.e., false-positive reaction ofE. coli host-derived proteins contaminating purified diagnostic antigen with human sera. The N (nucleocapsid) protein of Seoul virus causing haemorrhagic fever with renal syndrome (HFRS) was produced inE. coli BL21 (DE3), and used for the detection of N protein-specific antibodies in human sera. Using the N protein as a diagnostic antigen of HFRS, the false-positive reaction was cleared by merely mixing the test sera with the extract ofE. coli host strain not harboring expression plasmid.

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  1. R&D Center of Cheil Jedang, Corp., 522-1 Dokpyong-Ri, Majang-Myon, 467-810, Ichon-Si, Kyonggi-Do, Korea

    Kap Soo Noh, Jong Wan Kim, Suk Hoon Ha, Wang Don Yoo, Yeong Joong Jeon & Hyun Su Kim

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  1. Kap Soo Noh
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  2. Jong Wan Kim
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  3. Suk Hoon Ha
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  4. Wang Don Yoo
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  5. Yeong Joong Jeon
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  6. Hyun Su Kim
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Correspondence to Kap Soo Noh.

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Noh, K.S., Kim, J.W., Ha, S.H. et al. Clearance of false-positive antigen-antibody reactions of a diagnostic antigen produced inEscherichia coli with human sera. Biotechnol. Bioprocess Eng. 4, 63–65 (1999). https://doi.org/10.1007/BF02931917

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  • DOI: https://doi.org/10.1007/BF02931917

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