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Production of lyophilized culture ofLactobacillus acidophilus with preserving cell viability

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Abstract

Optimal lyophilization process was developed for manufacturing the dried product ofLactobacillus acidophilus with high cell viability. Three major factors, freezing rate, specific surface area of samples, and stabilizer type and their synergy were shown to play a crucial role in the development of an effective lyophilization process. Finally we found an optimal combination among three process parameters mentioned above: an exceptionally high cell survival percentage of 90% was achieved using the 8.28 cm−1 specific surface area of samples, slow freezing rate, and a stabilizer composition of 4% skim milk+1% glycerol +0.1% calcium chloride.

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Authors and Affiliations

  1. Bioprocess Eng. Lab., Hanhyo Institutes of Technology, Yusong, 305-309, Taejon, Korea

    Moo Heon Kang

  2. Biochemical Process Engineering R. U., Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yusong, P. O. Box 115, 305-600, Taejon, Korea

    Vibhor Saraswat, Jeewon Lee & Young-Hoon Park

Authors
  1. Moo Heon Kang
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  2. Vibhor Saraswat
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  3. Jeewon Lee
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  4. Young-Hoon Park
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Correspondence to Jeewon Lee.

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Kang, M.H., Saraswat, V., Lee, J. et al. Production of lyophilized culture ofLactobacillus acidophilus with preserving cell viability. Biotechnol. Bioprocess Eng. 4, 36–40 (1999). https://doi.org/10.1007/BF02931911

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