Skip to main content
SpringerLink
Log in

Molecular screening and characterization of antiviral potatoes

  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

Potato plants carrying theRy gene are extremely resistance to a number of potyviruses, but it is not known which variety expressed the resistance. In this investigation, combined classical and molecular techniques were used to identify virus resistance potatoes. Mechanical inoculation of 32 varieties of Korean potato cultivars, with potato virus Y (PVY), induced various symptoms, such as mosaic, yellowing, necrosis, mottle, vein claaring and vein bending. Different virus spreading patterns were observed, such as highly sensitive, moderate and resistant to PVYo inoculated leaves in different cultivars. From the results of double antibody sandwich-enzyme links immunosorbant assays (DAS-ELISA), coupled with reverse transcription polymerase chain reaction (RT-PCR),Winter valley andGolden valley were found to be highly susceptible and resistant cultivars to PVYo, respectively. TEM was used as a complementary method to conform the localization of the virus in leaf tissues. TEM detect virus particles inGolden valley, where, ELISA and RT-PCR were unable to detect theCP gene. However, the interior part of the tissues was severely deformed in PVYo infectedWinter valley, thanGolden valley. TheRy gene is involved in an induced response in PVYo infectedGolden valley plants. The methods described in this study could be applied for the screening and development of antiviral potatoes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Valkonen, J. P. T. (1994) Natural genes and mechanisms for resistance to viruses in cultivated and wild potato species (Solanum spp.).Plant Breed. 112: 1–16.

    Article  Google Scholar 

  2. Hinrichs, J., S. Berger, and J. G. Shaw (1998) A hypersensitive response-like mechanism is involved in resistance of potato plants bearing theRy(sto) gene to the potyviruses potato virus Y and tobacco etch virus.J. Gen. Virol. 79: 167–176.

    CAS  Google Scholar 

  3. Barker, H. (1993) Incidence of potato viras Y infection in seed and ware tubers of the potato cv. Record.Ann. Appl. Biol. 124: 179–183.

    Article  Google Scholar 

  4. Jones, D. A. C., J. A. T. Woodford, S. C. Main, D. Pallett, and H. Barker (1996) The role of volunteer potatoes in spread of potato virus YN in ware crops of cv. Record.Ann. Appl. Biol. 129: 471–478.

    Article  Google Scholar 

  5. De Bokx, J. A. and H. Huttinga (1981) Descriptions of plant viruses.Commonw. Mycol. Inst. Assoc. 242.

  6. Cockerham, G. (1943) Potato breeding for virus resistance.Ann. Appl. Biol. 30: 105–108.

    Article  Google Scholar 

  7. Fernandez-Northcote, E. N. (1983) Prospects for stability of resistance to potato virus Y. InResearch for the Potato in the Year 2000 (Hooker, W. J. ed.). Lima: International Potato Center. pp. 82.

    Google Scholar 

  8. Ross, H. (1952) Studies on mosaic resistance in the potato. InProceedings of the 1951 Conference on Potato Virus Diseases. Wageningen-Lisse, The Netherlands. pp. 40–47.

  9. Adams, S. E., R. A. C. Jones, and R. H. A. Coutts (1986) Expression of potato virus X resistant geneRx in potato leaf protoplasts.J. Gen. Virol. 67: 2341–2345.

    Article  CAS  Google Scholar 

  10. Jones, R. A. (1990) Strain group specific and virus specific hypersensitive reactions to infection with potyviruses in potato cultivars.Ann. Appl. Biol. 117: 93–105.

    Article  Google Scholar 

  11. Roh, K. S. and B. Y. Choi (2004) Sucrose regulates growth and activation of rubisco in tobacco leavesin vitro Biotechnol. Bioprocess Eng. 9: 229–235.

    Article  CAS  Google Scholar 

  12. Park, J. P., S. J. Lee, T. J. Park, K. B. Lee, J. S. Choi, S. Y. Lee, M. G. Kim, and B. H. Chung (2004) Microcontact printing of biotin for selective immobilization of streptavidin-fused proteins and SPR analysis.Biotechnol. Bioprocess Eng. 9: 137–142.

    Article  CAS  Google Scholar 

  13. Barker, H. and B. D. Harrison (1984) Expression of genes for resistance to potato virus Y in potato plants and protoplasts.Ann. Appl. Biol. 105: 539–545.

    Article  Google Scholar 

  14. Fang, Y. L., S. P. Dhital, K. H. Li, D. M. Khu, H. Y. Kim, Y. S. Song, and H. T. Lim (2005) Utilization of single nodal cuttings and therapies for eradicating doubleinfected potato virus (PLRV, PVY) fromin vitro plantlets of potato (Solanum tuberosum).J. Kor. Soc. Hort. Sci. 46: 119–125.

    CAS  Google Scholar 

  15. Nie, X. and R. P. Singh (2001) A novel usage of random primers for multiplex RT-PCR detection of virus and viroid in aphids, leaves, and tuvers.J. Virol. Methods 91: 37–49.

    Article  CAS  Google Scholar 

  16. Myslik, J. T. and A. Nassuth (2001) Rapid detection of viruses, transgenes, and mRNAs in small plant leaf samples.Plant Mol. Biol. Rep. 19: 329–340.

    Article  CAS  Google Scholar 

  17. Andre, N. D., C. Cesar, C. T. Antonio, and C. A. Antonio (2001) Resistance levels to two strains ofPotato virus Y (PVY) in transgenic potatoes cv. Achat.Hortic. Bras. 19: 348–350.

    Google Scholar 

  18. Horne, R. W. and P. Wildy (1963) Virus structure revealed by negative staining.Adv. Virus Res. 10: 101–170.

    Article  CAS  Google Scholar 

  19. Mori, K., R. Iriye, M. Hirata, and K. Takamizawa (2004) Quantification ofBacillus species in a wastewater treatment system by the molecular analyses.Biotechnol. Bioprocess Eng. 9: 482–489.

    Article  CAS  Google Scholar 

  20. Vidal, S., H. Cabrera, R. A. Andersson, A. Fredriksson, and J. P. Valkonen (2002) Potato gene Y-1 is an N gene homolog that confers cell death upon infection with potato virus Y.MPMI 15: 717–727.

    Article  CAS  Google Scholar 

  21. Hamalainen, J. H., V. A. Sorri, K. N. Watanable, C. Gebhardt, and J. P. T. Valkonen (1998) Molecular examination of a chromosome region that controls resistance to potato Y and A potyviruses in potato.Theor. Appl. Genet. 96: 1036–1043.

    Article  CAS  Google Scholar 

  22. Jones, J. B., J. P. Jones, R. E. Stall, and T. A. Zitter (1991)Compendium of Tomato Diseases. pp. 42–43. American Phytopathological Society, St. Paul, Minnesota, USA.

    Google Scholar 

  23. Bendahmane, A., K. Kanyuka, and D. C. Baulcombe (1999) The Rx gene from potato controls separate virus resistance and cell death responses.Plant Cell 11: 781–791.

    Article  CAS  Google Scholar 

  24. Hinrichs-Berger, J., M. Harfold, S. Berger, and H. Buchenauer (1999) Cytological responses of susceptible and extremely resistant potato plants to inoculation with potato virus Y.Physiol. Mol. Plant Pathol. 55: 143–150.

    Article  Google Scholar 

  25. Kosslak, R. M., M. A. Chamberlin, R. G. Palmer, and B. A. Bowen (1997) Programmed cell death in root cortex of soybean root necrosis mutants.Plant. J. 11: 729–745.

    Article  CAS  Google Scholar 

  26. Barker, H. (1996) Inheritance of resistance to potato viruses Y and A in progeny obtained from potato cultivars containing geneRy: evidence for new gene for extreme resistance to PVA.Theor. Appl. Genet. 93: 710–716.

    Article  CAS  Google Scholar 

  27. Brigneti, G., J. Garcia-Mass, and D. C. Baulcombe (1997) Molecular mapping of potato virus Y restance gene (Ry sto) in potato.Theor. Appl. Genet. 94: 198–203.

    Article  CAS  Google Scholar 

  28. Hamalainen, J. H., K. N. Watanable, J. P. T. Valkonen, A. Arihara, R. L. Plaisted, E. Pehu, L. Miller and S. A. Slack (1997) Mapping and marker-assisted selection for a gene for extreme resistance potato virus Y.Theor. Appl. Genet. 94: 192–197.

    Article  CAS  Google Scholar 

  29. Gebhardt, C. and J. P. Valkonen (2001) Organization of genes controlling disease resistance in the potato genome.Annu. Rev. Phytopathol. 39: 79–102.

    Article  CAS  Google Scholar 

  30. Leister, D., A. Ballvora, F. Salamini, and C. Gebhardt (1996) A PCR based approach for isolating pathogen resistance genes from potato with potential for wide application in plants.Nat. Genet. 14: 421–429.

    Article  CAS  Google Scholar 

  31. Yi, J. Y., H. W. Seo, S. W. Lee, M. S. Yang, and S. Y. Kim (2003) NPTH protein expression and resistance to bacterial disease in transgenic potato with lytic gene.J. Kor. Soc. Hort. Sci. 44: 187–191.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, College of Natural Sciences, Chosun University, 501-759, Gwangju, Korea

    Giriraj Tripathi, Hongxain Li, Jaekyun Park & Hyeonsook Cheong

  2. Department of Biomaterials, Graduated School, Chosun University, 501-759, Gwangju, Korea

    Yoonkyung Park

Authors
  1. Giriraj Tripathi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hongxain Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jaekyun Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yoonkyung Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hyeonsook Cheong
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hyeonsook Cheong.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tripathi, G., Li, H., Park, J. et al. Molecular screening and characterization of antiviral potatoes. Biotechnol. Bioprocess Eng. 11, 89–95 (2006). https://doi.org/10.1007/BF02931889

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02931889

Keywords

Search

Navigation