Abstract
Caco-2 cells (exhibiting characteristics of mature villus enterocytes) were used to determine bacteria (Salmonella enteritidis causing human gastroenteritis) — intestinal cell interactions. The interference of bacteria with the transepithelial electrical resistance (TEER) of filter-grown Caco-2 cells and the production of IL-8 after exposure of the cells toS. enteritidis 857 and/orLactobacillus strains (L. gasseri LF221 andL. rhamnosus BGT10) was evaluated. The strain 857 decreased TEER of filter-grown Caco-2 cells; in contrast, lactobacilli had a little or no effect. The effect ofS. enteritidis on the TEER decreased if Caco-2 cells were pre-incubated with lactobacilli. This strain induced high levels of IL-8 (which can lead to cell damage). Compared to the IL-8 synthesis after exposure of Caco-2 cells toS. enteritidis 857, simultaneous exposure of Caco-2 cells toS. enteritidis and lactobacilli inhibited the IL-8 synthesis after short recovery periods.
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This research was supported by theEuropean Community Marie-Curie Fellowship (HPMT-CT-2000-00005) and aFEMS Research Fellowship (2005-1) awarded to the first author.
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Fajdiga, S., Koninkx, J.F.J.G., Tooten, P.C.J. et al. Interference ofSalmonella enteritidis andLactobacillus spp. with IL-8 levels and transepithelial electrical resistance of enterocyte-like caco-2 cells. Folia Microbiol 51, 268–272 (2006). https://doi.org/10.1007/BF02931810
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DOI: https://doi.org/10.1007/BF02931810