Abstract
High levels of an aromatic nitrilase (about 37 µkat/L culture) were induced inFusarium solani O1 after transfer of the mycelium from a rich medium into a medium with 20 mmol/L picolinonitrile. The mycelium was entrapped in lense-shaped particles consisting of a polyvinyl alcohol/polyethylene glycol copolymer (LentiKats®). The cell-free extract was immobilized by hydrophobic binding onto a Buty1 Sepharose column. The enzyme was useful for the mild hydrolysis of nicotinonitrile, isonicotinonitrile and benzonitrile.
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Vejvoda, V., Kaplan, O., Klozová, J. et al. Mild hydrolysis of nitriles byFusarium solani strain O1. Folia Microbiol 51, 251–256 (2006). https://doi.org/10.1007/BF02931806
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DOI: https://doi.org/10.1007/BF02931806