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Improved isolation of outer root sheath cells from human hair follicles and their proliferation behavior under serum-free condition

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Abstract

Hair follicle is a small but very complex and dynamic miniorgan of the human body. It is easy to isolate and culture mesenchymal cells but not epithelial cells of hair follicle. It is necessary for intact and healthy outer root sheath (ORS) cells to be isolated and cultured. In this study we developed an appropriate isolation method to yield 6.4±0.75×104 cells/hair follicle, which is about 9-fold comparing to our previous data. This yield was achieved by modifications such as different kinds of enzyme uses, fragmentation, and mechanical stimuli. Especially we detected that the different kinds of isolation enzyme could affect proliferation of ORS cells during primary culture. In addition, bovine pituitary extract (BPE) was needed for ORS cells to proliferate and to form colonies under serum-free, feeder layer-free culture condition, but type I collagen as a substratum did not have any positive effect. Moreover, ORS cells under BPE-added condition contained stem/progenitor cells expressing β1-integrin. CK19, and CD34. These results can provide useful cell culture information, not only in the study of hair biology but also in the field of tissue engineering and cell therapy for the treatment of alopecia.

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Authors and Affiliations

  1. Department of Chemical and Biochemical Engineering, Dongguk University, 100-715, Seoul, Korea

    Bo-Young Yoo, Hee-Hoon Yoon & Jung-Keug Park

  2. Biomedical Research Institute, Lifecord Inc., 442-749, Suwon, Korea

    Youn-Ho Shin & Young-Jin Kim

  3. Department of Pathology, Chung-Ang University College of Medicine, 156-756, Seoul, Korea

    Kye-Yong Song

  4. SJ HairHair Clinics, 135-887, Seoul, Korea

    Sung-Joo Hwang

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  1. Bo-Young Yoo
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  2. Youn-Ho Shin
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  4. Young-Jin Kim
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  7. Jung-Keug Park
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Correspondence to Jung-Keug Park.

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These two authors equally contribute to this paper.

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Yoo, BY., Shin, YH., Yoon, HH. et al. Improved isolation of outer root sheath cells from human hair follicles and their proliferation behavior under serum-free condition. Biotechnol. Bioprocess Eng. 12, 54–59 (2007). https://doi.org/10.1007/BF02931804

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  • DOI: https://doi.org/10.1007/BF02931804

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