Abstract
Human umbilical cord blood (UCB)-derived multipotent stem cells are regarded as valuable sources for cell transplantation and cell therapy. These cells, under appropriate culture conditions, can differentiate into a variety of cell lineages such as osteoblasts. chondrocyles, adipocytes, and neuronal cells. Based on their largeex vivo expansion capacity as well as their differentiation potential, UCB-derived multipotent stem cells may become a suitable source for clinical transplantation in tissue engineering and regenerative medicine. All modern protocols involve the use of cytokines with chemotherapy in order to increase the circulation of stem cells in the blood. Because UCB, in general, produce less cytokine, or have a lower frequency of cytokine producing cells compared to adult stem cells, further research in cytokines related to the cell proliferation, cellular adhesion and cell migration is necessary to improve the understanding of the basic mechanisms of stem cell mobilization. This paper gives an overview of the cytokines produced by UCB-derived multipotent stem cells, and strongly suggests that cytokine induction and signal transduction is important for the differentiation of these cells.
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Reyes, M., T. Lund, T. Lenvik, D. Aguiar, L. Koodie, and C. M. Verfaillie (2001) Purification andex vivo expansion of postnatal human marrow mesodermal progenitor cells.Blood 98: 2615–2625.
Pittenger, M. F., A. M. Mackay, S. C. Beck, R. K. Jaiswal, R. Douglas, J. D. Mosca, M. A. Moorman, D. W. Simonetti, S. Craig, and D. R. Marshak (1999) Multilineage potential of adult human mesenchymal stem cells.Science 284: 143–147.
Rubinstein, P., C. Carrier, A. Scaradavou, J. Kurtzberg, J. Adamson, A. R. Migliaccio, R. L. Berkowitz, M. Cabbad, N. L. Dobrila, R. E. Rosenfield, and C. E. Stevens (1998) Outcomes among 562 recipients of placental-blood transplants from unrelated donors.N. Engl. J. Med. 339: 1565–1577.
Gluckman, E., V. Rocha, W. Arcese, G. Michel, G. Sanz, K. W. Chan, T. A. Takahashi, J. Ortega, A. Filipovich, F. Locatelli, S. Asano, F. Fagioli, M. Vowels, A. Sirvent, J. P. Laporte, K. Tiedemann, S. Amadori, M. Abecassis, P. Bordigoni, B. Diez, P. J. Shaw, A. Vora, M. Caniglia, F. Garnier, I. Ionescu, J. Garcia, G. Koegler, P. Rebulla, and S. Chevret (2004) Factors associated with outcomes of unrelated cord blood transplant: guidelines for donor choice.Exp. Hematol. 32: 397–407.
Barker, J. N., and J. E. Wagner (2003) Umbilical cord blood transplantation: current practice and future innovations.Crit. Rev. Oncol. Hematol. 48: 35–43.
Rocha, V., J. Cornish, E. L. Sievers, A. Filipovich, F. Locatelli, C. Peters, M. Remberger, G. Michel, W. Arcese, S. Dallorso, K. Tiedemann, A. Busca, K. W. Chan, S. Kato, J. Ortega, M. Vowels, A. Zander, G. Souillet, A. Oakill, A. Woolfrey, A. L. Pay, A. Green F. Garnier, I. Ionescu, P. Wernet, G. Sirchia P. Rubinstein, S. Chevret, and E. Gluckman (2001) Comparison of outcomes of unrelated bone marrow and umbilical cord blood transplants in children with acute leukemia.Blood 97:2969–2971.
Kim, D. H., K. H. Yoo, K. S. Choi, J. Choi, S. Y. Choi, S. E. Yang, Y. S. Yang, H. J. Im, K. H. Kim, H. L. Jung, K. W. Sung, and H. H. Koo (2005) Gene expression profile of cytokine and growth factor during differentiation of bone marrow-derived mesenchymal stem cell.Cytokine 31: 119–126.
Liu, C. H., and S. M. Hwang (2005) Cytokine interactions in mesenchymal stem cells from cord blood,Cytokine 32: 270–279.
Kogler, G., T. F. Radke, A. Lefort, S. Sensken, J. Fischer, R. V. Sorg, and P. Wernet (2005) Cytokine production and hematopoiesis supporting activity of cord blood-derived unrestricted somatic stem cells.Exp. Hematol. 33: 573–583.
Kortesidis, A., A. Zannettino, S. Isenmann, S. Shi, T. Lapidot, and S. Gronthos (2005) Stromal-derived factor-1 promotes the growth, survival, and development of human bone marrow stromal stem cells.Blood 105: 3793–3801.
Wang, B., D. T. Hendricks, F. Wamunyokoli, and M. I. Parker (2006) A growth-related oncogene/CXC chemokine receptor 2 autocrine loop contributes to cellular proliferation in esophageal cancer.Cancer Res. 66: 3071–3077.
Singh, U. P., S. Singh, P. N. Boyaka, J. R. McGhee, and J. W. Lillard, Jr. (2004) Granulocyte chemotactic protein-2 mediates adaptive immunity in part through IL-8Rbeta interactions.J. Leukoc. Biol. 76: 1240–1247.
Fernandes, J. L., R. L. Mamoni, J. L. Orford, C. Garcia, A. P. Selwyn, O. R. Coelho, and M. H. Blotta (2004) Increased Th 1 activity in patients with coronary artery disease.Cytokine 26: 131–137.
Kaneider, N. C., C. M. Reinisch, S. Dunzendorfer, C. Meierhofer, A. Djanani, and C. J. Wiedermann (2001) Induction of apoptosis and inhibition of migration of inflammatory and vascular wall cells by cerivastatin.Atherosclerosis 158: 23–33.
Matsui, T., T. Akahoshi, R. Namai, A. Hashimoto, Y. Kurihara, M. Rana, A. Nishimura, H. Endo, H. Kitasato, S. Kawai, K. Takagishi, and H. Kondo (2001) Selective recruitment of CCR6-expressing cells by increased production of MIP-3 alpha in rheumatoid arthritis.Clin. Exp. Immunol. 125: 155–161.
Boot, R. G., M. Verhoek, M. de Fost, C. E. Hollak, M. Maas, B. Bleijlevens, M. J. van Breemen, M. van Meurs, L. A. Boven, J. D. Laman, M. T. Moran, T. M. Cox, and J. M. Aerts (2004) Marked elevation of the chemokine CCL18/PARC in Gaucher disease: a novel surrogate marker for assessing therapeutic intervention.Blood 103: 33–39.
Von Luttichau, I., M. Notohamiprodjo, A. Wechselberger, C. Peters, A. Henger, C. Seliger, R. Djafarzadeh, R. Huss, and P. J. Nelson (2005) Human adult CD34-progenitor cells functionally express the chemokine receptors CCR1, CCR4, CCR7, CXCR5, and CCR10 but not CXCR4.Stem Cells Dev. 14: 329–336.
Lisignoli, G., A. Piacentini, S. Toneguzzi, F. Grassi, B. Cocchini, A. Ferruzzi, G. Gualtieri, and A. Facchini (2000) Osteoblasts and stromal cells isolated from femora in rheumatoid arthritis (RA) and osteoarthritis (OA) patients express IL-11, leukaemia inhibitory factor and oncostatin M,Clin. Exp. Immunol. 119: 346–353.
Kang, X. Q., W. J. Zang, L. J. Bao, D. L. Li, T. S. Song, X. L. Xu, and X. J. Yu (2005) Fibroblast growth factor-4 and hepatocyte growth factor induce differentiation of human umbilical cord blood-derived mesenchymal stem cells into hepatocytes.World J. Gastroenterol. 11: 7461–7465.
Grimaud, E., F. Blanchard, C. Charrier, F. Gouin, F. Redini, and D. Heymann (2002) Leukaemia inhibitory factor (LIF) is expressed in hypertrophic chondrocytes and vascular sprouts during osteogenesis.Cytokine 20: 224–230.
Son B. R., L. A. Marquez-Curtis, M. Kucia, M. Wysoczynski, A. R. Turner, J. Ratajczak, M. Z. Ratajczak, and A. Janowska-Wieczorek (2006) Migration of bone marrow and cord blood mesenchymal stem cellsin vitro is regulated by stromal-derived factor-1-CXCR4 and hepatocyte growth factor-c-met axes and involves matrix metalloproteinases.Stem Cells 24: 1254–1264.
Fiedler, J., F. Leucht, J. Waltenberger, C. Dehio, and R. E. Brenner (2005) VEGF-A and PIGF-1 stimulate chemotactic migration of human mesenchymal progenitor cells.Biochem. Biophys. Res. Commun. 334: 561–568.
Jang, Y. K., D. H. Jung, M. H. Jung, D. H. Kim, K. H. Yoo, K. W. Sung, H. H. Koo, W. Oh, Y. S. Yang, and S. E. Yang (2000) Mesenchymal stem cells feeder layer from human umbilical cord blood forex vivo expanded growth and proliferation of hematopoietic progenitor cells.Ann. Hematol. 85: 212–225.
Kiepe, D., S. Ciarmatori, A. Haarmann, and B. Tonshoff (2006) Differential expression of IGF system components in proflierating vs. differentiating growth plate chondrocytes: the functional role of IGFBP-5.Am. J. Physiol. Endocrinol. Metab. 290: E363-E371.
Schling, P., C. Rudolph, S. Heimerl, S. Fruth, and G. Schmitz (2006) Expression of tumor necrosis factor alpha and its receptors during cellular differentiation.Cytokine 33: 239–245.
Zannettino, A. C., C. A. Holding, P. Diamond, G. J. Atkins, P. Kostakis, A. Farrugia, J. Gamble, L. B. To, D. M. Findlay, and D. R. Haynes (2005) Osteoprotegerin (OPG) is localized to the Weibel-Palade bodies of human vascular endothelial cells and is physically associated with von Willebrand factor.J. Cell Physiol. 204: 714–723.
Kralisch, S., J. Klein, U. Lossner, M. Bluher, R. Paschke, M. Stumvoll, and M. Fasshauer (2005) Proinflammatory adipocytokines induce TIMP-1 expression in 3T3-L1 adipocytes.FEBS Lett. 579: 6417–6422.
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Hwang, S.H., Kim, M.H., Yang, I.H. et al. Analysis of cytokines in umbilical cord blood-derived multipotent stem cell. Biotechnol. Bioprocess Eng. 12, 32–38 (2007). https://doi.org/10.1007/BF02931800
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DOI: https://doi.org/10.1007/BF02931800