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Toll-like receptor gene polymorphism and its relationship with somatic cell concentration and natural bacterial infections of the mammary gland in sheep

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Abstract

Possible correlation between Toll-like receptor (TLR)-gene mutations and the susceptibility of the mammary gland to bacterial infections and also the associate breed-dependent aspects of somatic cell concentration (SCC), bacterial infection and TLR-gene mutations in sheep are described. In Polish Lowland Sheep (PLS), milk samples exceeding the level of 500/µL (i.e. 5 × 105 per mL) of SCC were recorded almost twice more frequently than in Polish Heath Sheep (PHS) (40 and 22.3 %, respectively). The frequency of bacterial infections was also found in a similar ratio (20 and 12.7 %, respectively). During detection of the TLR-gene mutation we recorded 2 alleles ofTLR1, 6 alleles ofTLR2 and 10 alleles ofTLR4 genes in PHS sheep, while PLS sheep possessed 2, 4 and 6 alleles, respectively. Statistical analyses revealed a relationship between the specified TLR alleles, SCC and the frequency of incidence of bacterial inflammations of mammary gland. The data may serve as a benchmark for further study of TLR-gene mutation-dependent predisposition of mammary gland defensive cells to recognize the pathogen properly and initiate the immunological response, and may help in identifying one of the markers of natural resistance against sheep mastitis.

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Abbreviations

CI:

confidence interval

OR:

odds ratio

PAMP:

pathogen associated molecular patterns

PCR-SSCP:

polymerase-chain reaction-single strand conformational polymorphism analysis

PHS:

Polish Heath Sheep (ewes)

PLS:

Polish Lowland Sheep (ewes)

SCC:

somatic cell concentration

SSC:

sub-clinical mastitis occurrence

TLR:

Toll-like receptor

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Authors and Affiliations

  1. Department of Genetics and Animal Breeding, Warsaw Agricultural University — SGGW, 02-786, Warsaw, Poland

    W. P. Świderek, J. Gruszczyńska & D. Witkowska

  2. Laboratory of Biomedical Microbiology and Immunology, University of Veterinary Medicine, 040 13, Košice, Slovakia

    M. R. Bhide & I. Mikula

  3. Institute of Neuroimmunology, Slovak Academy of Sciences, 842 45, Bratislava, Slovakia

    K. Soltis & I. Mikula

Authors
  1. W. P. Świderek
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  2. M. R. Bhide
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  3. J. Gruszczyńska
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  4. K. Soltis
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  5. D. Witkowska
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  6. I. Mikula
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Correspondence to W. P. Świderek.

Additional information

The work was supported bySlovak Grant Agency for Science (VEGA 1/3239/06, VEGA 2/4125/26) and byMinistry Education of the Slovak Republic (AV 802/2002, AV 1140/2004) and by theAD Centrer of Excellence (Slovak Academy of Sciences).

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Świderek, W.P., Bhide, M.R., Gruszczyńska, J. et al. Toll-like receptor gene polymorphism and its relationship with somatic cell concentration and natural bacterial infections of the mammary gland in sheep. Folia Microbiol 51, 647–652 (2006). https://doi.org/10.1007/BF02931633

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  • DOI: https://doi.org/10.1007/BF02931633

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