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The mechanisms of activation of normal human serum complement byEscherichia coli strains with K1 surface antigen

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Abstract

TenE. coli K1 strains isolated from the urine of children with urinary tract infections were sensitive to the bactericidal action of normal human serum (NHS). The role of the particular mechanisms of complement activation was determined in the process of killing these strains, showing variable sensitivity to the bactericidal action of NHS; three mechanisms of activation of human complement were observed. Important role of alternative pathway activation in the bactericidal action of NHS againstE. coli K1 strains independent of the classical and lectin pathways was not established.

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Abbreviations

EGTA:

glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

NeuNAc:

5-acetamido-3,5-dideoxy-d-glycero-d-galacto-2-nonulosonic acid

HS50°C:

human serum with inhibited alternative pathway of complement activation

HSMgEGTA:

human serum with inhibited classical and lectin pathways of complement activation

LPS:

lipopolysaccharide(s)

NHS:

normal human serum

OM:

outer membrane

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Authors and Affiliations

  1. Institute of Genetics and Microbiology University of Wroclaw, 51-148, Wroclaw, Poland

    G. Bugla-Płoskońska, K. Karpińska & W. Doroszkiewicz

  2. Department of Biology and Medical Parasitology, Medical University, 50-367, Wrocław, Poland

    A. Cisowska & S. Jankowski

Authors
  1. G. Bugla-Płoskońska
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  2. A. Cisowska
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  3. K. Karpińska
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  4. S. Jankowski
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  5. W. Doroszkiewicz
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Correspondence to G. Bugla-Płoskońska.

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Bugla-Płoskońska, G., Cisowska, A., Karpińska, K. et al. The mechanisms of activation of normal human serum complement byEscherichia coli strains with K1 surface antigen. Folia Microbiol 51, 627–632 (2006). https://doi.org/10.1007/BF02931630

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  • DOI: https://doi.org/10.1007/BF02931630

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