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The impact of acrylonitrile and bioaugmentation on the biodegradation activity and bacterial community structure of a topsoil

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Abstract

The analysis of the bacterial community within the soil using DGGE showed acrylonitrile (ACN) could lead to the selection of significantly similar communities. Moreover,Rhodococcus sp. AJ270 was successfully established in the soil community. High GC G+-bacteria also responded positively to ACN addition. Bioaugmentation or carbon addition had no impact on the rate or degree of ACN degradation. ACN could be readily degraded by the soil bacteria, however, the community structure was significantly affected by its addition as well as by the addition of carbon orRhodococcus sp. AJ270. The bioaugmentation of the soil with this strain was successful, in that the organism became established within the community. ACN addition to a soil produces statistically significant changes in the bacterial community.

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Abbreviations

ACN:

acrylonitrile

CFU:

colony-forming unit(s)

DGGE:

denaturing gradient gel electrophoresis

PBS:

phosphate-buffered saline

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Author notes

  1. N. J. Garton

    Present address: Department of Infection, Immunity & Inflammation, University of Leicester, PO Box 138, LE1 9HN, Leicester, UK

Authors and Affiliations

  1. Biomolecular and Biomedical Research Centre, School of Applied Sciences, Northumbria University, Newcastle-upon-Tyne, UK

    J. Baxter

  2. School of Applied Sciences, Ellison Building, Northumbria University, NE1 8ST, Newcastle-upon-Tyne, UK

    S. P. Cummings

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  1. J. Baxter
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  3. S. P. Cummings
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Correspondence to S. P. Cummings.

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Baxter, J., Garton, N.J. & Cummings, S.P. The impact of acrylonitrile and bioaugmentation on the biodegradation activity and bacterial community structure of a topsoil. Folia Microbiol 51, 591–597 (2006). https://doi.org/10.1007/BF02931624

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  • DOI: https://doi.org/10.1007/BF02931624

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