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The fission yeast ortholog of eIF3a subunit is not functional inSaccharomyces cerevisiae

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Abstract

TheSchizosaccharomyces pombe eIF3a ortholog (SpeIF3a) was shown to be unable to substitute forS. cerevisiae eIF3a (SceIF3a) in its essential function in the initiation of translation. Overproduction of SpeIF3a altered the distribution of SceIF3a but formation of the endogenous eIF3 complex was not affected. SpeIF3a was found to be more tightly bound toS. cerevisiae ribosomes than SceIF3a and other eIF3 subunits (eIF3g, eIF3i, eIF3j). The host cells displayed aberrant morphology and altered chitin deposition. SpeIF3a probably competes with SceIF3a for binding to either ribosomes or yet to be identified substrates.

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Authors and Affiliations

  1. Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20, Prague, Czechia

    I. Malcová-Janatová, Z. Koubek, K. Malínská, R. Raková & J. Hašek

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  1. I. Malcová-Janatová
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  2. Z. Koubek
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  3. K. Malínská
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  4. R. Raková
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  5. J. Hašek
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Correspondence to J. Hašek.

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This work was supported by grants of theCzech Science Foundation 204/02/1424 and 204/05/0838 to the corresponding author and byInstitutional Research Concept no. AV 0Z 5020 903.

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Malcová-Janatová, I., Koubek, Z., Malínská, K. et al. The fission yeast ortholog of eIF3a subunit is not functional inSaccharomyces cerevisiae . Folia Microbiol 51, 555–564 (2006). https://doi.org/10.1007/BF02931620

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  • DOI: https://doi.org/10.1007/BF02931620

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