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The effect of nalidixic acid on growth and reproductive events in nucleocytosolic and chloroplast compartments in the algaScenedesmus quadricauda

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Abstract

The courses of rRNA accumulation, DNA replication, and nuclear division were followed both in the chloroplast and the nucleocytosolic compartments during the cell cycle in synchronized populations of the chlorococcal algaScenedesmus quadricauda. Control and nalidixic acid-treated cultures were compared. Nalidixic acid (150 mg/L) was added either at the beginning of the cell cycle or consecutively during the cell cycle to subcultures transferred into the dark. If the inhibitor was applied at the beginning of the cell cycle, chloroplast DNA did not replicate and nucleoids did not divide. Chloroplast division, however, was coordinated in a timely fashion with cytokinesis even under conditions of blocked chloroplast DNA replication. While the growth rate was slowed down, the courses of reproductive processes in the nucleocytosolic compartment were not affected and their timing and the number of rounds were coordinated with growth rate as in the control culture. The rate of cytosolic rRNA synthesis was lower but no apparent effect was seen on the amount of rRNA that accumulated during the cell cycle. In contrast, lower levels of chloroplast rRNA were found at the end of the cell cycle compared with the control culture. Experiments in which cells were transferred to the dark during the cell cycle showed that the inhibitor affected none of the reproductive events in the nucleocytosolic compartment. In the chloroplast compartment, DNA replication was inhibited in inhibitor-treated cultures, but was unaffected in controls. The chloroplast nucleoids themselves divided even in the presence of the inhibitor, reducing their DNA content to a level which corresponded to that in freshly formed control daughter cells.

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Authors and Affiliations

  1. Cell Cycle Laboratory, Department of Autotrophic Microorganisms, Institute of Microbiology, Academy of Sciences of the Czech Republic, 379 81, Třeboň, Czechia

    V. Zachleder

  2. Plant Life System Laboratory, Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, Japan

    S. Kawano

  3. Department of Algology, Institute of Botany, Academy of Sciences of the Czech Republic, Třeboň, and Research Centre for Environmental Chemistry and Ecotoxicology, Masaryk University, Kamenice Brno, Czechia

    V. Cepák

  4. Graduate School of Science, University of Tokyo, Hongo, Tokyo, Japan

    T. Kuroiwa

Authors
  1. V. Zachleder
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  2. S. Kawano
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  3. V. Cepák
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  4. T. Kuroiwa
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Correspondence to V. Zachleder.

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This work was supported by grant no. 206/02/1438 of theGrant Agency of the Czech Republic, and byInstitutional Research Concept no. AV 0Z 502 0903.

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Zachleder, V., Kawano, S., Cepák, V. et al. The effect of nalidixic acid on growth and reproductive events in nucleocytosolic and chloroplast compartments in the algaScenedesmus quadricauda . Folia Microbiol 49, 441–451 (2004). https://doi.org/10.1007/BF02931607

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