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Enteroaggregative and cell-detachingEscherichia coli strains among polish children with and without diarrhea

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Abstract

To determine the association of enteroaggregative (EAEC) and cell-detaching (CDEC)Escherichia coli with diarrhea of unknown origin among children from Wrocław (Poland),E. coli strains isolated from stool specimens of children with diarrhea were examined for mannose-resistant adherence to HEp-2 cells. EAEC were isolated from 10 of 39 (26%) children examined with diarrhea and 4 of 20 (20%) age-matched controls. CDEC were present in 14 (36%) cases of diarrhea and 7 (35%) healthy subjects. Cell-detaching activity was distinctly associated with hemolysin production. Among hemolytic CDEC strains cytotoxic necrotizing factor 1 (CNF1) synthesis prevailed among isolates obtained from cases of diarrhea (57%) in comparison with isolates obtained from healthy controls (14.3%). Although neither EAEC nor CDECE. coli strains were associated with diarrhea of children in this setting, there were differences among EAEC and CDEC strains isolated from children with and without diarrhea.

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Abbreviations

CDEC:

cell-detachnigEscherichia coli

CDT:

cytolethal distending toxin

CNF1:

cytotoxic necrotizing factor 1

EAEC:

enteroaggregativeEscherichia coli

Hlyα:

E. coli α-hemolysin

MRHA:

mannose-resistant hemagglutination

ShET1, ShET2:

Shigella spp. enterotoxins

SLT2:

Shiga-like toxin 2

References

  • Albert M.J., Faruque S.M., Faruque A.S., Bettelheim K.A., Neogi P.K., Bhuiyam N.A., Kaper J.P.: Controlled study of cytolethal distending toxin-producingE. coli infections in Bangladeshi children.J.Clin.Microbiol. 34, 717–719 (1996).

    PubMed  CAS  Google Scholar 

  • Baldwin T.J., Knutton S., Seller S.L., Hernandez H.A., Aitken A., Williams P.H.: EnteroaggregativeEscherichia coli strains secrete a heat-labile toxin antigenically related toE. coli hemolysin.Infect.Immun. 60, 2092–2095 (1992).

    PubMed  CAS  Google Scholar 

  • Beutin L., Montenegro M.A., Ørskov I., Ørskov F., Prada J., Zimmermann S., Stephan R.: Close association of verotoxin (Shiga-like toxin) production with enterohemolysin production in strains ofEscherichia coli.J.Clin.Microbiol. 27, 2559–2564 (1989).

    PubMed  CAS  Google Scholar 

  • Blanco M., Blanco J.E., Blanco J., Alonso M.P., Balsalobre C., Mourino M., Madrid C., Juarez A.: Polymerase chain reaction for detection ofEscherichia coli strains producing cytotoxin necrotizing factor type 1 and type 2 (CNF1 and CNF2).J.Microbiol.Meth. 26, 95–101 (1996).

    Article  CAS  Google Scholar 

  • Bogyiová E., Siegfried L., Kmeťová M., Šándorčinová Z., Liptáková A., Biroš E.: Occurrence and genetic association of selected virulence factors in clinicalEscherichia coli isolates.Folia Microbiol. 47, 73–77 (2002).

    Article  Google Scholar 

  • Cravioto A., Gross R.J., Scotland S.M., Rowe B.: An adhesive factor found in strains ofEscherichia coli belonging to the traditional infantile enteropathogenic serotypes.Curr.Microbiol. 3, 95–99 (1979).

    Article  Google Scholar 

  • Czeczulin J.R., Balepur S., Hicks S., Phillips A., Hall R., Kothary M.H., Navarro-Garcia F., Nataro J.P.: Aggregative adherence fimbria II, a second fimbrial antigen mediating aggregative adherence in enteroaggregativeEscherichia coli.Infect.Immun. 65, 4135–4145 (1997).

    PubMed  CAS  Google Scholar 

  • De Rycke J., Gonzales E.A., Blanco J., Oswald F., Blanco M., Boivin R.: Evidence for two types of cytotoxic necrotizing factor in human and animal clinical isolates ofEscherichia coli.J.Clin.Microbiol. 28, 694–699 (1990).

    PubMed  Google Scholar 

  • De Rycke J., Mazars P., Nougayrede J.P., Tasca C., Boury M., Herault F., Valette A., Oswald E.: Mitotic block and delayed lethality in HeLa cells exposed toEscherichia coli BM2-1 producing cytotoxic necrotizing factor type 1.Infect. Immun. 64, 1694–1705 (1996).

    PubMed  Google Scholar 

  • De Rycke J., Milon A., Oswald E.: NecrotoxicEscherichia coli (NTEC): two emerging categories of human and animal pathogens.Vet.Res. 30, 221–233 (1999).

    PubMed  Google Scholar 

  • Duguid J.P., Clegg S., Wilson M.J.: The fimbrial and nonfimbrial hemagglutinins ofEscherichia coli.J.Med.Microbiol. 12, 213–227 (1979).

    Article  PubMed  CAS  Google Scholar 

  • Echeverria P., Serichantalerg O., Changchawalit S., Baudry B., Levine M.M., Ørskov F., Ørskov I.: Tissue culture-adherentEscherichia coli in infantile diarrhea.J.Infect.Dis. 165, 141–143 (1992).

    PubMed  CAS  Google Scholar 

  • Elliot S.J., Srinivas S., Albert M.J., Albert M.J., Alam K., Robins-Browne R.M., Gunzburg S.T., Mee J.B., Chang B.J.: Characterization of the roles of hemolysin and other toxins in enteropathy caused by α-hemolyticEscherichia coli linked to human diarrhea.Infect.Immun. 66, 2040–2051 (1998).

    Google Scholar 

  • Eslava C., Navarro-Garcia F., Czeczulin J.R., Henderson I.R., Cravioto A., Nataro J.P.: Pet, an autotransporter enterotoxin from enteroaggregativeEscherichia coli.Infect.Immun. 66, 3155–3163 (1998).

    PubMed  CAS  Google Scholar 

  • Fabrega A., Ferreira P., Silva R., Brinkley C., Scaletsky A.I.: Cell-detachingEscherichia coli (CDEC) strains from children with diarrhea: identification of a protein with toxigenic activity.FEMS Microbiol.Lett. 217, 191–197 (2002).

    Article  Google Scholar 

  • Gioppo N.M., Elias W.P., Vidotto M.C., Linhares R.E., Saridakis H.O., Gomes T.A., Trabulsi L.R., Pelayo J.S.: Prevalence of HEp-2 cell-adherentEscherichia coli and characterization of enteroaggregativeE. coli and chain-like adherentE. coli isolates from children with and without diarrhea, in Londrina, Brazil.FEMS Microbiol.Lett. 190, 293–298 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Gomes T.A., Abe C.M.: Detection of HeLa cell-detaching activity and α-hemolysin production in enteroaggregativeEscherichia coli strains isolated from feces Brazilian children.J.Clin.Microbiol. 33, 3364 (1995).

    PubMed  CAS  Google Scholar 

  • Gomes T.A., Vieira M.A., Abe C.M., Rodrigues D., Griffin P.M., Ramos S.R.: Adherence patterns and adherence-related DNA sequences inEscherichia coli isolates from children with and without diarrhea in São Paulo City, Brazil.J.Clin.Microbiol. 36, 3609–3613 (1998).

    PubMed  CAS  Google Scholar 

  • Gonzales R., Diaz C., Marino M., Clorat R., Pequeneze M., Perez-Schael I.: Age-specific prevalence ofEscherichia coli with localized and aggregative adherence in Venezuclan infants with acute diarrhea.J.Clin.Microbiol. 35, 1103–1107 (1997).

    Google Scholar 

  • Gunzburg S.T., Chang B.J., Elliot S.T., Burke V., Gracey M.: Diffuse and enteroaggregative patterns of adherence of entericEscherichia coli isolated from aboriginal children from the Kimberley region of Western Australia.J.Infect.Dis. 167, 755–758 (1993).

    PubMed  CAS  Google Scholar 

  • Henderson I.R., Czeczulin J., Eslava C., Noriega F., Nataro J.P.: Characterization of Pic, a secreted protease ofShigella flexneri and enteroaggregativeEscherichia coli.Infect.Immun. 67, 5587–5596 (1999).

    PubMed  CAS  Google Scholar 

  • Huppertz H.I., Rutkowski S., Aleksic S., Karch H.: Acute and chronic diarrhea and abdominal colic associated with enteroaggregativeEscherichia coli in young children living in Western Europe.Lancet 349, 1660–1662 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Island M.D., Cui X., Foxman B., Marrs C.F., Stamm W.E., Stapleton A.E., Warren J.W.: Cytotoxicity of hemolytic, cytotoxic necrotizing factor 1-positive and -negativeEscherichia coli to human T24 bladder cells.Infect.Immun. 66, 3384–3389 (1998).

    PubMed  CAS  Google Scholar 

  • Law D., Chart H.: EnteroaggregativeEscherichia coli.J.Appl.Microbiol. 84, 685–697 (1996).

    Article  Google Scholar 

  • Liptaková A., Siegfried L., Podracká L’., Sabol M., Sehnálková H., Bogyiova E., Rosocha J., Kmeťová M., Kerestešová H., Kotulová D.: Detection of shiga toxins, intimin and enterohemolysin inEscherichia coli strains isolated from children in Eastern Slovakia.Folia Microbiol. 47, 185–188 (2002).

    Article  Google Scholar 

  • Marques L.R., Abe C.M., Griffin P.M., Gomes T.A.: Association between α-hemolysin production and HeLa cell-detaching activity in fecal isolates ofEscherichia coli.J.Clin.Microbiol. 33, 2707–2709 (1996).

    Google Scholar 

  • Morabito S., Karch H., Mariani-Kurkdjian P., Schmidt H., Minelli F., Bingen E., Caprioli A.: Enteroaggregative, Shiga-toxin-producingEscherichia coli O111:H2 associated with an outbreak of hemolytic uremic syndrome.J.Clin.Microbiol. 36, 840–842 (1998).

    PubMed  CAS  Google Scholar 

  • Nandy R.K., Barari S.K., Ghose A.C.: Expression of antigenically distinct fimbriae with hemagglutination and HeLa cell adherence properties by an enteroaggregativeEscherichia coli strain belonging to the enteropathogenic serogroup.FEMS Microbiol. Lett. 9, 143–150 (1994).

    CAS  Google Scholar 

  • Nataro J.P., Yikang D., Maneval D.R., German A.L., Martin W.C., Levine M.M.: Aggregative adherence fimbriae I of entero-aggregativeEscherichia coli mediate adherence to HEp-2 cells and hemagglutination of human erythrocytes.Infect.Immun. 60, 2297–2304 (1992).

    PubMed  CAS  Google Scholar 

  • Okeke J.N., Lamikanra A., Steinrück H., Kaper J.B.: Characterization ofEscherichia coli strains from cases of childhood diarrhea in provincial Southwestern Nigeria.J.Clin.Microbiol. 38, 7–12 (2000).

    PubMed  CAS  Google Scholar 

  • Okeke I.N., Steinruck H., Kanack K.J., Elliot S.J., Sundstrom L., Kaper J.B., Lamikanara A.: Antibiotic-resistant cell-detachingEscherichia coli strains from Nigerian children.J.Clin.Microbiol. 40, 301–305 (2002).

    Article  PubMed  Google Scholar 

  • Savarino S.J., Fasano A., Robertson P.C., Levine M.M.: EnteroaggregativeEscherichia coli elaborate a heat-stable enterotoxin demonstratesin vitro rabbit intestinal model.J.Clin.Invest. 87, 1450–1455 (1991).

    Article  PubMed  CAS  Google Scholar 

  • Suttrop N., Flöer B., Schmittler H., Seeger W., Bhakdi S.: Effects ofEscherichia coli hemolysin on endothelial cell function.Infect.Immun. 58, 3796–3801 (1990).

    Google Scholar 

  • Suzart S., Guth B.E., Pedroso M.Z., Okafor U.M., Gomes T.A.: Diversity of surface structures and virulence genetic markers among enteroaggregativeEscherichia coli (EAEC) strains with and without the EAEC DNA probe sequence.FEMS Microbiol.Lett. 201, 163–168 (2001).

    Article  PubMed  CAS  Google Scholar 

  • Vila J., Vargas M., Henderson I.R., Gascon J., Nataro J.P.: EnteroaggregativeEscherichia coli virulence factors in traveler’s diarrhea strains.J.Infect.Dis. 182, 1780–1783 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Yamamoto T., Endo S., Yokota T., Echeverria P.: Characteristics of adherence of enteroaggregativeEscherichia coli to human and animal mucosa.Infect.Immun. 59, 3722–3739 (1991).

    PubMed  CAS  Google Scholar 

  • Yamamoto T., Wakisaka N., Nakae T.: A novel cryohemagglutinin associated with adherence of enteroaggregativeEscherichia coli.Infect.Immun. 65, 3478–3484 (1997).

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, University of Medicine, 50-368, Wroclaw, Poland

    B. M. Sobieszczańska

  2. Department of Microbiology, National Veterinary Research Institute, 24-100, Puławy, Poland

    J. Osek

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  1. B. M. Sobieszczańska
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  2. J. Osek
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Correspondence to B. M. Sobieszczańska.

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Sobieszczańska, B.M., Osek, J. Enteroaggregative and cell-detachingEscherichia coli strains among polish children with and without diarrhea. Folia Microbiol 48, 823–828 (2003). https://doi.org/10.1007/BF02931520

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  • DOI: https://doi.org/10.1007/BF02931520

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