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Detection of aflatoxigenic fungi in feeds using the PCR method

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Abstract

The possibility of using the polymerase chain reaction (PCR) to speed up and specify the detection of aflatoxigenic fungi isolated from feed was investigated. The method, applied to 2 genes encoding the biosynthesis of aflatoxins (apa-2 andver-1), was optimized on two collection cultures (Aspergillus flavus CCM F-108 andA. parasiticus CCM F-550). The specificity of the optimized PCR method was proved on collection cultures of different kinds of fungi. Fifty feed samples out of which 18 showed positive findings of aflatoxigenic fungi on anAspergillus Flavus and Parasiticus Agar (AFPA) medium were tested. Isolated strains ofAspergillus strains were verified using the PCR method; its reaction products were detected in 1% agarose gel by electrophoresis. The results almost exclusively matched those gained from the AFPA medium.

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Authors and Affiliations

  1. Department of Biological and Biochemical Sciences, University of Pardubice, 530 03, Pardubice, Czechia

    I. Zachová, J. Vytřasová, M. Pejchalová & L. Červenka

  2. Institute for Hygiene and Pathology of Animal Nutrition, Veterinary Faculty, University of Ljubljana, 1000, Ljubljana, Slovenia

    G. Tavčar-Kalcher

Authors
  1. I. Zachová
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  2. J. Vytřasová
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  3. M. Pejchalová
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  4. L. Červenka
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  5. G. Tavčar-Kalcher
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Additional information

This work was supported by theMinistry of Education. Youth and Sports of the Czech Republic, project no. 253 100 002. Support from program 201/005 funded by theCzech-Slovenian Intergovernmental S and T Cooperation Programe for 2001–2002 project proposal is gratefully acknowledged.

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Zachová, I., Vytřasová, J., Pejchalová, M. et al. Detection of aflatoxigenic fungi in feeds using the PCR method. Folia Microbiol 48, 817–821 (2003). https://doi.org/10.1007/BF02931519

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  • DOI: https://doi.org/10.1007/BF02931519

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