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Significance ofHerbaspirillum seropedicae inoculation and/or straw amendment on growth and dinitrogen fixation of wheat using15N-dilution method

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Abstract

The effect ofHerbaspirillum seropedicae inoculation and/or maize straw (0, 5 and 10 Mg/hm2) amendment on the growth and N2 fixation of wheat was determined in pot experiments using15N-dilution method. Inoculation resulted in accumulation of fixed nitrogen, and % N from atmosphere being 24.6 and 26.5 % in wheat shoot and grain, respectively. Straw amendment reduced % Natm to 16.1 and 20.2 % at high straw level (10 Mg/hm2). Rational nitrogen fertilization (180 kg N/hm2) completely inhibited N2 fixation byH. seropedicae inoculation. Bacterial inoculation increased dry shoot and grain yield up to 23 and 31 %, respectively. The highest levels of shoot and grain dry mass (46.5 and 42.4 %) were obtained by N-fertilization in both inoculated and uninoculated plants. Total shoot and grain N-yield increased irrespective of organic matter amendment by inoculation up to 9 and 25 %, respectively. N-fertilized plants recorded a maximum increase in N-yield (57 and 51 %).H. seropedicae was reisolated from inoculated wheat histosphere after harvesting (90 d from sowing). Neither organic matter nor mineral nitrogen applications had any marked effect on bacterial total counts colonizing wheat histosphere. Moreover, no symptoms of mottled stripe disease were observed on leaves and stems of inoculated plants.

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Abbreviations

Nfix :

N2 fixed (mg per pot)

Ntot :

total N-yield (mg per pot)

DM:

dry matter

ae:

atom excess

atm:

derived from atmosphere

fer:

derived from fertilizer

fix:

inoculated (fixing) plants

inorg:

inorganic

non:

uninoculated (nonfixing) plants

om:

derived from organic matter

s:

derived from soil

(+):

in plant with organic matter

(−):

in plant without organic matter

References

  • Abdel Wahab A.M., El Komy H.M., Sholkamy M.A.: Para-nodule induction in wheat with 2,4-D and its infection withHerbaspirillum spp. and the possible role of 2,4-D in enhancing BNF. Abstr. p. 185,10th Internat. Colloquium Optimization of Plant Nutrition, Cairo 2000.

  • Abo Arkob M.M.: GenusPseudomonas, pp. 382–390 inBiological Control of Plant Diseases (ISBN 977-281-113-8). Academic Books Publishers, Cairo 2000.

    Google Scholar 

  • Baldani J.I., Baldani V.L.D., Seldin L., Dobereiner J.: Characterization ofHerbaspirillum seropedicae gen.nov., a root associated nitrogen-fixing bacterium.Internat.J.Syst.Bacteriol.36, 86–93 (1986).

    CAS  Google Scholar 

  • Baldani V.L.D., Baldani J.I., Dobereiner J.: Inoculation of rice plants with the endophytic diazotrophsHerbaspirillum seropedicae andBurkholderia spp.Biol.Fertil.Soil30, 485–491 (2000).

    Article  Google Scholar 

  • Boddey R.M., de Oliveira O.C., Urquigia V.M., Reis de Olivares F.L., Baldani V.L.D., Dobereiner J.: Biological nitrogen fixation associated with sugarcane and rice: contribution and prospects for improvement.Plant & Soil174, 195–209 (1995).

    Article  CAS  Google Scholar 

  • Caballero-Mellado J., Carcano Mantiel M.G., Mascarua-Esparza M.A.: Field inoculation of wheat withAzospirillum brasilense under temperate climate.Symbiosis13, 243–253 (1992).

    Google Scholar 

  • Cavalcante V.A., Dobereiner J.: A new acid-tolerant nitrogen fixing bacterium associated with sugarcane.Plant & Soil108, 23–31 (1988).

    Article  Google Scholar 

  • Deka Boruah H.P., Dileep Kumar B.S.: Plant disease suppression and growth promotion by a fluorescentPseudomonas strain.Folia Microbiol.47, 137–144 (2002).

    Article  Google Scholar 

  • Dobereiner J., Baldani V.L.D., Olivares F., de Reis V.M.: Endophytic diazotrophs: the key to BNF in gramineous plants, pp. 395–408 in N.A. Hegazi, M. Fayez, M. Monib (Eds):Nitrogen Fixation with Non-Legumes. American University in Cairo Press, Cairo 1994.

    Google Scholar 

  • El-Komy H.M.: Plant hydrolytic enzymes (carboxymethylcellulase and polygalacturonase) in maize roots inoculated simultaneously with VA mycorrhiza and endophytic nitrogen-fixing bacteria.J.Union Arab Biol.8, 89–99 (1999).

    Google Scholar 

  • El-Komy H.M.: Survival of and wheat-root colonization by alginate encapsulatedHerbaspirillum spp.Folia Microbiol.46, 25–30 (2001).

    Article  CAS  Google Scholar 

  • El-Komy H.M., Abdel-Wahab A.M.: Effect of simultaneous inoculation ofAzospirillum andRhizobium spp. on growth, nodulation and nitrogen fixation of two legumes using the15N-isotope dilution technique (IDT) and the difference methods (DM).Acta Microbiol.Polon.47, 283–296 (1998).

    Google Scholar 

  • El-Komy H.M., Moharram T.M.M., Safwat M.S.A.: Effect ofAzospirillum inoculation on growth and N2 fixation of maize subjected to different levels of FYM using15N-dilution method, pp. 49–59 inProc. 7th Internat. Symp. Nitrogen Fixation with Non-Legumes. Kluwer Academic Publishers, Dordrecht-Boston-London 1998.

    Google Scholar 

  • Fages J.:Azospirillum inoculants and field experiments, pp. 87–110 in Y. Okon (Ed.): Azospirillum-Plant Associations. CRS Press, Boca Raton (Fla) 1994.

    Google Scholar 

  • Fried M., Middleboe V.: Measurement of amount of nitrogen fixed by a legume crop.Plant & Soil47, 713–715 (1997).

    Article  Google Scholar 

  • Fu A.H., Burris R.H.: Ammonium inhibition of nitrogenase activity inHerbaspirillum seropedicae.J.Bacteriol.171, 3168–3175 (1989).

    PubMed  CAS  Google Scholar 

  • Gillis M., Kerters B., Hoste D.J., Kroppenstedt R.M., Stephan M.P., Teixeira R.S., Dobereiner J., De Ley J.:Acetobacter diazotrophicus sp.nov., a nitrogen fixing bacterium associated with sugarcane.Internat.J.Syst.Bacteriol.39, 361–364 (1989).

    Google Scholar 

  • Gillis M., Dobereiner J., Pot B., Goor M., Falsen E., Hoste B., Reinhold B., Kersters K.: Taxonomic relationships between [Pseudomonas]rubrisbalbicans, some clinical isolates (EF group 1),Herbaspirillum seropedicae and [Aquaspirillum]autotrophium, pp. 292–294 in M. Polsinelli, R. Materassi, M. Vincenzini (Eds):Nitrogen Fixation. Kluwer Academic Publishers, Dordrecht 1991.

    Google Scholar 

  • Hamdia M.A., El-Komy H.M.: Effect of salinity, gibberellic acid andAzospirillum inoculation on growth and nitrogen uptake ofZea mays.Biol.Plant.40, 109–120 (1998).

    Article  Google Scholar 

  • Hegazi N.A., Monib M., Amer H.A., Shoker E.: Response of maize plants to inoculation with azospirilla and (or) straw amendment in Egypt.Can.J.Microbiol.29, 888–894 (1983).

    Article  Google Scholar 

  • James E.K., Gyaneshwar P., Natarajan M., Reddy P.M., Olivares F.L., Ladha J.: Infection and colonization of rice seedlings by plant growth-promoting bacteriumHerbaspirillum seropedicae Z67.Molec.Plant Microbiol.Interact.15, 894–906 (2002).

    Article  CAS  Google Scholar 

  • Kassen G., Pedrosa F.O., Souza E.M., Funayama S., Rigo L.U.: Effect of nitrogen compounds on nitrogenase activity inHerbaspirillum seropedicae SMR1.Can.J.Microbiol.43, 887–891 (1997).

    Article  Google Scholar 

  • Kaushik R., Saxena A.K., Tilak K.V.B.R.: Selection and evaluation ofAzospirillum brasilense strains growing at a sub-optimum temperature in rhizocoenosis with wheat.Folia Microbiol.46, 327–332 (2001).

    Article  CAS  Google Scholar 

  • Kirchhof G., Eckert B., Stoffels M., Baldani J.I., Reis V.M., Hartmann A.:Herbaspirillum frisingense sp.nov., a new nitrogen fixing bacterial species occurs in C4-fibre plants.Internat.J.Syst.Evol.Microbiol.51, 157–168 (2001).

    CAS  Google Scholar 

  • Olivares F.L., Baldani V.L.D., Baldani I., Dobereiner J.: Ecology ofHerbaspirillum spp. and ways of infection and colonization of cereals with these endophytic diazotrophs, pp. 357–358 in N.A. Hegazi, M. Fayez, M. Monib (Eds):Nitrogen Fixation with Non-Legumes. American University in Cairo Press, Cairo 1994.

    Google Scholar 

  • Palleroni J.N.: Family I.Pseudomonadaceae, pp. 141–199 in N.R. Krieg, J.G. Holt (Eds):Bergey’s Manual of Systematic Bacteriology, Vol. 1. Williams & Wilkins, Baltimore 1984.

    Google Scholar 

  • Palus J.A., Borneman J., Ludden P.W., Triplett E.W.: A diazotrophic bacterial endophyte isolated from stems ofZea mays L. andZea luxuriansIltis andDoebley.Plant & Soil186, 135–142 (1996).

    Article  CAS  Google Scholar 

  • Perreira J.A.R., Baldani J.I.: Selection ofAzospirillum andHerbaspirillum seropedicae strains to inoculate rice and maize plants, pp. 220–221 ofInternat. Symp. Sustainable Agriculture for the Tropics, the Role of BNF. Angra dos Reis, Rio de Janeiro 1995.

    Google Scholar 

  • Pimentel J.P., Olivares F., Pitard R.M., Urquiaga S., Akiba F., Dobereiner J.: Dinitrogen fixation and infection of grass leaves byPseudomonas rubrisubalbicans andHerbaspirillum seropedicae.Plant & Soil137, 61–65 (1991).

    Article  Google Scholar 

  • Rennie R.J., Defreitas J.R., Ruschel A.P., Vose P.B.:15N-isotope dilution to quantify nitrogen fixation associated with Canadian and Brazilian wheat.Can.J.Bot.61, 1667–1671 (1982).

    Google Scholar 

  • Roper M.M., Ladha J.K.: Biological N2 fixation by heterotrophic and phototrophic bacteria in association with straw.Plant & Soil174, 211–224 (1995).

    Article  CAS  Google Scholar 

  • Shaban G.M., Fadl-Alla E.M., El-Komy H.M.: Role of cellulose decomposing fungi onAzospirillum maize association.Egypt. J.Microbiol.32, 309–327 (1997).

    Google Scholar 

  • Triplett E.W.: Diazotrophic endophytes: progress and respects for nitrogen fixation in monocots.Plant & Soil186, 29–38 (1996).

    Article  CAS  Google Scholar 

  • Zambre M.A., Konde B.K., Sonar K.R.: Effect ofAzotobacter chroococum andAzospirillum brasilense under graded levels of nitrogen on growth and yield of wheat.Plant & Soil79, 61–67 (1984).

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Botany, Faculty of Science and, Minia University, 615 19, Minia, Egypt

    H. M. A. El-Komy & A. M. A. Hetta

  2. Department of Microbiology, Faculty of Agriculture, Minia University, 615 19, Minia, Egypt

    O. A. O. Saad

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  1. H. M. A. El-Komy
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  2. O. A. O. Saad
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  3. A. M. A. Hetta
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El-Komy, H.M.A., Saad, O.A.O. & Hetta, A.M.A. Significance ofHerbaspirillum seropedicae inoculation and/or straw amendment on growth and dinitrogen fixation of wheat using15N-dilution method. Folia Microbiol 48, 787–793 (2003). https://doi.org/10.1007/BF02931515

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  • DOI: https://doi.org/10.1007/BF02931515

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