Abstract
We performed detailed phenotypic analysis of theisw2Δ strains of the W303 genetic background and compared its results with those obtained previously in BY-derived genetic background. Shmoolike morphology was observed in theisw2Δ strain of α-mating type of the BY strains, but not in its W303-derived counterpart. On the other hand, derepression ofa-specific genes in theisw2Δ (MATα) strain was observed in both genetic backgrounds, although to a different extent. Unlike in BY-derived strain hyperactivation of the Ras2/cAMP pathway reduced invasiveness of theisw2Δ strain (MATα) of the W303 background. Sensitivity to Calcofluor White indicating a cell wall-integrity defect was significantly increased in theisw2Δ strains of the W303 background in contrast to BY-derived strains. Our data indicate that the effects of theisw2 deletion strongly depend on the background in which the deletion is made.
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This work was supported by grant A502 0102 of theGrant Agency of the Academy of Sciences of the Czech Republic and by theInstitutional Research Concept no. AV 0Z 502 0903.
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Trachtulcová, P., Frýdlová, I., Janatová, I. et al. The W303 genetic background affects theisw2Δ mutant phenotype inSaccharomyces cerevisiae . Folia Microbiol 48, 745–753 (2003). https://doi.org/10.1007/BF02931508
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DOI: https://doi.org/10.1007/BF02931508